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Showing: 6601 - 6630 of 148509 results

  6. pAC-BETA

    Plasmid
    #53272
    Purpose
    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.
    Insert
    crtE, crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53272-plasmid-map-sequence-id-83327

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  13. pDEST-CMV mCherry-GFP-LC3B WT

    Plasmid
    #123230
    Purpose
    Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123230-plasmid-map-sequence-id-244035

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  14. T4 Lysozyme L99A WT*

    Plasmid
    #18476
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C54T C97A L99A WT*
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only
    18476-plasmid-map-sequence-id-202584

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  17. pU6T7

    Plasmid
    #71462
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)
    Depositor
    Willie Mark
    Article
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    human U6/phage T7
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    71462-plasmid-map-sequence-id-128762

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  19. pDAC627

    Plasmid
    #195240
    Purpose
    Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195240-plasmid-map-sequence-id-382948

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  21. CoV2-Spike-D614G

    Plasmid
    #177960
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177960-plasmid-map-sequence-id-348179

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  23. pTP1005

    Plasmid
    #104160
    Purpose
    Bacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104160-plasmid-map-sequence-id-200057

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  24. TFORF1500

    Plasmid
    #143960
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RBL2 (RBL2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143960-plasmid-map-sequence-id-404618

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Showing: 6601 - 6630 of 148509 results