-
Plasmid#192289PurposeExpresses GST- and mCherry-tagged HRS FYVE in e ColiDepositorInsertHRS FYVE domain
UseTagsGST-TEV-mCherryExpressionBacterialMutationPromotertacAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
miReporter-CAG
Plasmid#82478PurposemicroRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.DepositorInsertH2B-mCherry and H2B-Citrine. HygroR
UseTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Plasma membrane-targeted optoPLD
Plasmid#140114PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expressionDepositorInsertPhospholipase D
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
Plasmid#198417PurposeExpresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
H52-pUCM-hNGN2-inv
Plasmid#110492PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology armDepositorInsertsUseCRISPR, Cre/Lox, and TALENTagsExpressionMammalianMutationPromoterCAG, EF-1 alpha, and TRE3GAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plasma membrane-targeted optodPLD
Plasmid#140061PurposeEncodes CRY2-mCherry-dPLD-P2A-CIBN-CAAX for mammalian expressionDepositorInsertPhospholipase D
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationH170A (catalytic dead)PromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBWB162
Plasmid#140634PurposepBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFM689
Plasmid#74635Purposealias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection markerDepositorInsertsUseTagsExpressionYeastMutationPromoternoneAvailable sinceJune 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMU-PDESr
Plasmid#61206PurposeFluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtPDLP1
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMU-MTUBr
Plasmid#61196PurposeFluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoterDepositorInsertMAP-MBD
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Endoplasmic reticulum-targeted optoPLD
Plasmid#140060PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expressionDepositorInsertPhospholipase D
UseTagsCIBN, CRY2, P2A, Sac1(TM), and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Endosome-targeted optoPLD
Plasmid#140056PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expressionDepositorInsertPhospholipase D
UseTags2xFYVE, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMU-AUTr
Plasmid#61204PurposeFluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoterDepositorInsertLC3(ATG8)
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMU-ACTFr
Plasmid#61191PurposeFluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoterDepositorInsertAtFimbrin1-ABD
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKHR7
Plasmid#74624Purposea cmlc2::mCherry (red heart) reporter gene was inserted at the 3’end of pKHR4 mcs, that resides within donor fragments produced by I-SceI digestion. Genbank accession number is KU144824.DepositorInsertcmlc2::mCherry
UseZebrafish expressionTagsExpressionMutationPromotercmlc2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR83
Plasmid#90211PurposeExpresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Golgi-targeted optoPLD
Plasmid#140058PurposeEncodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expressionDepositorInsertPhospholipase D
UseTagsCIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
UPmCh
Plasmid#161004Purposeubiquitous expression of PCP-mCherryDepositorInsertPCP-mCherry
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMU-GAr
Plasmid#61172PurposeFluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under AtUBQ10 promoterDepositorInsertGmMAN49-mCherry
UseTagsExpressionPlantMutationPromoterAtUBQ10Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TPr
Plasmid#61190PurposeFluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoterDepositorInsertAtTIP1-1
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only