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pJH2644

Plasmid

#179632

Purpose

Pnmr-1::TetanusToxin-UrSL-wCherry unc-54 3' UTR C.elegans AVA/E/D premoter IN and others expression of TetanusToxin-UrSL-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

TetanusToxin

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGAN101

Plasmid

#131123

Purpose

Plasmid for overexpression of prion domains to induce prions

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Tags

mKate2

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC4_dual-MTS-3x-Set I

Plasmid

#162762

Purpose

Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_ABEmax_IVT

Plasmid

#171761

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

ABEmax

Use

Vector for in-vitro transcription

Tags

Expression

Mutation

Promoter

T3 promoter

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miRNT

Plasmid

#177808

Purpose

A nontargeting miRNA based on the pre-miR- 30a structure (Qiu et al., 2008) has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-NT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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WFS1 P724L-Flag-pcDNA3

Plasmid

#13012

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed P 724 to L

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only
pEasy Flox

Plasmid

#11725

Depositor

Klaus Rajewsky

Article

Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

Insert

pEasyFlox

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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CMV-Cx35-Halo-C-end

Plasmid

#71512

Purpose

Mammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAT15485_Hypa-ABE8e

Plasmid

#174123

Purpose

Expresses ABE8e fused with Hypa-SpCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

Hypa-ABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMTP130 (pTA106_TnsABC(Tn6900))

Plasmid

#164261

Purpose

Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Insert

tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1

Use

Tags

Expression

Bacterial

Mutation

(tnsA) Changed Alanine 125 to Aspartic acid

Promoter

Lactose

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Expression

Mutation

Promoter

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pGEM-sgMUC1_dual-MTS-3x-Set I

Plasmid

#162763

Purpose

Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

Depositor

Antony K Chen

Article

Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

Insert

sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-H-Luc_mito

Plasmid

#113930

Purpose

Plasmid encoding the mictochonfrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_mito

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-U6-sgRNA-CMV-puro

Plasmid

#169450

Purpose

backbone for sgRNA expression

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

sgRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJM126

Plasmid

#49339

Purpose

bacterial expression of RFA1, RFA2, and RFA3

Depositor

Steven Brill

Article

He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

Inserts

RFA3 (RFA3 Budding Yeast)
RFA2 (RFA2 Budding Yeast)
RFA1 (RFA1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only
ER2-hfYFP

Plasmid

#186518

Purpose

Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSCT-GAL93-PSPC1

Plasmid

#46321

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

PSPC1 (PSPC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-Sport6 Baf60b

Plasmid

#24723

Depositor

Benoit Bruneau

Article

Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

Insert

Baf60b (Smarcd2 Mouse)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLN380 (FuGW-S(CEBPB)p-mKate2)

Plasmid

#105178

Purpose

synthetic promoter CEBPB drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(CEBPB)p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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