Skip to main content
Addgene
Showing: 4381 - 4400 of 5448 results

  1. TFORF0394

    Plasmid
    #141601
    Purpose
    Lentiviral vector for overexpressing the ZNF7 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF7 (ZNF7 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141601-plasmid-map-sequence-id-409101

    Enlarge

  8. pGL3-Basic-ZC3H12D_PromoterMUT

    Plasmid
    #153067
    Purpose
    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter
    Depositor
    Silvia Monticelli
    Article
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutagenesis to disrupt 3 BHLHE4040 binding sites.…
    Promoter
    None
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    153067-plasmid-map-sequence-id-304643

    Enlarge

  9. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid
    #155064
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155064-plasmid-map-sequence-id-308583

    Enlarge

  10. pLCHKO_TK1_HPRT1_Lb_5

    Plasmid
    #155063
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155063-plasmid-map-sequence-id-308581

    Enlarge

  11. pLCHKO_TK1_HPRT1_Lb_3

    Plasmid
    #155061
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    155061-plasmid-map-sequence-id-308580

    Enlarge

  12. pLCHKO_Ptbp_ex8_1

    Plasmid
    #155081
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155081-plasmid-map-sequence-id-308533

    Enlarge

  13. pLCHKO_Ptbp_ex8_4

    Plasmid
    #155084
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155084-plasmid-map-sequence-id-308527

    Enlarge

  14. pLCHKO_TK1_HPRT1_Lb_2

    Plasmid
    #155060
    Purpose
    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155060-plasmid-map-sequence-id-308523

    Enlarge

  15. pLCHKO_Ptbp_ex8_2

    Plasmid
    #155082
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155082-plasmid-map-sequence-id-308510

    Enlarge

  19. pKK-RNAi-nucCHERRYmiR-FLAG-TEV

    Plasmid
    #105809
    Purpose
    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with N-terminal FLAG.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    RNAi; Flp-in competent
    Tags
    FLAG-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105809-plasmid-map-sequence-id-203432

    Enlarge

Showing: 4381 - 4400 of 5448 results