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pTol2CG NBT:NRGIIIa_R551Q:polyA

Plasmid

#193013

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa R551Q patient variant in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa R551Q (NRG1 Human)

Use

Transposon transgenesis

Tags

Expression

Mammalian

Mutation

ENST00000287842, NM_013956, c.1652G>A, p.(Arg5…

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T (DM#111)

Plasmid

#15030

Depositor

Douglas Melton

Article

Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

Insert

beta-globin 5' and 3' UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pSP64TS (DM#117)

Plasmid

#15034

Depositor

Douglas Melton

Article

Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

Insert

beta-globin 5' and 3' UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pTLNX

Plasmid

#49032

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

Expression

Mutation

Promoter

SP6

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAIP4_1TJ6

Plasmid

#234167

Purpose

Heterologous protein expression of sprouty-related, EVH1 domain-containing protein 1 (Spred-1) in Escherichia coli

Depositor

Christopher Bahl

Insert

1TJ6 (spred1 Xenopus tropicalis)

Use

Tags

10x HisTag-Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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x.Setd7

Plasmid

#87133

Purpose

mammalian expression of Setd7

Depositor

Francesca Spagnoli

Article

Kofent et al Development. 2016 Oct 1;143(19):3573-358

Insert

Setd7 (setd7.L Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

simian CMV IE94

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only
C245

Plasmid

#174351

Purpose

Template for making mRNA for overexpressing frog RPL3 (C-HA) in frog oocytes

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

rpl3.L (rpl3.L Frog)

Use

In vitro transcription template

Tags

HA

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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OSR1-pCS2+MT

Plasmid

#30491

Depositor

Gerhart Ryffel

Article

Drews et al BMC Dev Biol. 2011 . 11():5.

Insert

OSR1 (OSR1 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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xfz3/pCS2+

Plasmid

#16327

Depositor

Peter Klein

Article

Deardorff et al Development. 2001 Oct . 128(19):3655-63.

Insert

frizzled-3 (fzd3.L Frog)

Use

Vertebrate expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pCS2 xIGF1

Plasmid

#24967

Depositor

Edward De Robertis

Article

Pera et al Dev Cell. 2001 Nov . 1(5):655-65.

Insert

insulin-like growth factor 1 (igf1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pTLNXA7

Plasmid

#49033

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter and N-terminal chicken alpha7 signal sequence

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

chicken alpha7 signal sequence

Expression

Mutation

Promoter

SP6

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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C205

Plasmid

#174359

Purpose

For expressing siRNA-resistant frog ePAB in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

ePAB

Use

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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p029 End-1::EnR

Plasmid

#11029

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Dominant inhibitory GATA-binding protein End1::En…

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pCiVSP-SD64TF

Plasmid

#80332

Purpose

Expresses Ciona VSP in Xenopus Oocyte

Depositor

Yasushi Okamura

Article

Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

Insert

Ciona intestinalis voltage-sensing phosphatase (vsp Ciona intestinalis)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

Promoter

SP6

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-pGBD

Plasmid

#26735

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Rac (rac1 Frog)

Use

Reporter for rac

Tags

GFP

Expression

Mammalian

Mutation

Rac binding domain of rhotekin

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-RFP

Plasmid

#26753

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Centrin 4 (cetn4 Frog)

Use

Reporter for basal body

Tags

monomeric RFP

Expression

Mutation

Promoter

Available since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pTol2_NBT:Cas9green; erbb2_gRNA171

Plasmid

#199337

Purpose

pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

NBT
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)(FRT)EYFP

Plasmid

#31304

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)(FRT)EYFP

Use

Cre/Lox; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCSCre2

Plasmid

#31308

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

Cre recombinase (cre bacteriophage P1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)EYFP

Plasmid

#31306

Depositor

Gerhart Ryffel

Article

Ryffel et al Nucleic Acids Res. 2003 Apr 15. 31(8):e4

Insert

ECFP(loxP)EYFP

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge