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pCSJ125
Plasmid#104344PurposeExpress yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertMDH2
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSJ95
Plasmid#104343PurposeExpress yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertFBP1
UseTags3xFLAGExpressionYeastMutationPromoterAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH511
Plasmid#102665PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDW115
Plasmid#70062Purposeartificial transcription template "pause ablation"DepositorInsertlambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
UseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGPS21loxFRTNeo
Plasmid#27180DepositorInsertlox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
UseIn vitro transposionTagsExpressionMutationPromoterAvailable sinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNH050
Plasmid#42156DepositorInsertCFP-RT-CFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH07
Plasmid#70063Purposeartificial transcription template "distal pause"DepositorInsertlambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
UseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNH051
Plasmid#42157DepositorInsertGFP-RT-GFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtAwoH
Plasmid#65017PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH09
Plasmid#70064Purposeartificial transcription template "terminator insertion"DepositorInsertlambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'
UseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHBJT032
Plasmid#225178PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (TetR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-cMYC
Plasmid#216510PurposeExpresses MYC in human cells under a tetracycline/doxycycline-inducible promoterDepositorInsertcMYC
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO2te
Plasmid#210243Purposemedium-copy plasmid for bacterial expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO4te
Plasmid#210245Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUdO1te
Plasmid#210242Purposehigh-copy plasmid for bacterial expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDT4
Plasmid#199120Purposescrambled promoter circular DNA templateDepositorInsertsynthetic transcription template
UseTagsExpressionBacterialMutationmutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …PromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_5a5a.10
Plasmid#178933PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_5a7_pKD46.10
Plasmid#178937PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_5a9.10
Plasmid#178936PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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