Showing: 421 - 440 of 591 results
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Plasmid#128953Purposeexpresses PiuD in E.coliDepositorInsertPutative outer membrane receptor for iron transport (PiuD)
UseTags6xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-105)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailabilityAcademic Institutions and Nonprofits only -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117AvailabilityAcademic Institutions and Nonprofits only -
pPPC030.306
Plasmid#171151PurposeMevalonate production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mvaES
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117AvailabilityAcademic Institutions and Nonprofits only -
pTEF1-UDH
Plasmid#166679PurposeYeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertUDH
UseSynthetic BiologyTagsExpressionYeastMutationPromoterTEF1AvailabilityAcademic Institutions and Nonprofits only -
pEXPA10-NLS-3xmCitrine
Plasmid#192523PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertEXPANSIN A10 (EXPA10 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterEXPA10AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
UseTags8xHis and EGFPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
UseTags6xHis and EGFPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pS848∙CsR
Plasmid#199240PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7AvailabilityAcademic Institutions and Nonprofits only -
pSEVA424::tsi5
Plasmid#192960PurposeExpresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organismsDepositorInserttsi5
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
UseTags9xhis + TEV tagExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::D1141A
Plasmid#192963PurposeTest the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGLDepositorInsertTse5_D1141A
UseTags9xhis + TEV tagExpressionBacterialMutationD1141APromoterAvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1-Pct5.1-crRNA(dadR)
Plasmid#191634PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)DepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pS44i8GH-1
Plasmid#207525PurposeConditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pS44i8GM
Plasmid#207527PurposeConditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpRDepositorInsertoriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
UseCRISPR; Pseudomonas vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pS548∙CsR
Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7AvailabilityAcademic Institutions and Nonprofits only -
pS648∙CsR
Plasmid#197860PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Gm resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPEM7AvailabilityAcademic Institutions and Nonprofits only -
P20_992-TetR
Plasmid#97094PurposeExpresses TetR in the presence of Pseudomonas fluorescence sigma factor ECF20_992DepositorInsertTetR
UseTagsExpressionBacterialMutationPromoterP20_992AvailabilityAcademic Institutions and Nonprofits only -
P20_992-MxiE
Plasmid#97099PurposeExpresses MxiE in the presence of Pseudomonas fluorescence sigma factor ECF20_992DepositorInsertMxiE
UseTagsExpressionBacterialMutationPromoterP20_992AvailabilityAcademic Institutions and Nonprofits only -
P20_992-deGFP
Plasmid#97095PurposeExpresses GFP in the presence of Pseudomonas fluorescence sigma factor ECF20_992DepositorInsertdeGFP
UseTagsExpressionBacterialMutationPromoterP20_992AvailabilityAcademic Institutions and Nonprofits only
Showing: 421 - 440 of 591 results