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Plasmid#159280PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven human PDL1/CD274 (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInsertHuman PDL1/CD274 (CD274 Human)
UseAAV; S1 knockin donor vectorTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.eGFP.WPRE.rBG
Plasmid#105542PurposeAAV expression of EGFP from CB7 promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterCB7Available sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.S72A
Plasmid#106176PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72APromoterhSynapsinAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40
Plasmid#100855PurposeAAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoterDepositorInsertjRGECO1b
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS6
Plasmid#170857PurposeAAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Nd1
Plasmid#191523PurposeExpression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vectorDepositorInsertv8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HyPer3
Plasmid#119183PurposeAAV expression vector with CMV promoter driving expression of the hydrogen peroxide-sensitive fluorescent biosensor HyPer3.DepositorInsertHyPer3
UseAAVTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_Cre
Plasmid#201198PurposeAAV vector for cre expression under EF1 promoterDepositorInsertscre
EF1alpha promoter
UseAAVTagsExpressionBacterial and MammalianMutationnuclear targeting crePromoterN/A and hEF1alpha promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CB7.CI.mCherry.WPRE.RBG
Plasmid#105544PurposeAAV expression of mCherry from CB7 promoterDepositorHas ServiceAAV9InsertCB7-CI-mCherry
UseAAVTagsExpressionMammalianMutationPromoterCB7Available sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn-FLEX.iGABASnFR.mRuby3
Plasmid#112169PurposeGABA SensorDepositorInsertiGABASnFR
UseAAVTagsmRuby3ExpressionMutationPromoterSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shControl
Plasmid#180386PurposeProducing AAV that encodes negative control shRNA with miR-E backboneDepositorInsertshControl
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-Tyr
Plasmid#135616PurposeDonor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locusDepositorInsertEcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn1.Twitch2B.WPRE.SV40
Plasmid#100040PurposeAAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signalingDepositorHas ServiceAAV1InsertTwitch2B
UseAAVTagsExpressionMammalianMutationPromoterhSyn1Available sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-SAGFP
Plasmid#127100PurposeSA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.DepositorInsertSA-GFP reporter
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184V
Plasmid#106199PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-STAT3
Plasmid#195575PurposeExpresses STAT3DepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterCMV enhancer and promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-myrSNAP
Plasmid#60098PurposeExpresses membrane-targeted SNAP(f)-tag and histone-GFP, non-conditionalDepositorInsertsSNAP-tag
histone-GFP
UseAAV and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-VTKS3
Plasmid#170854PurposeAAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IV-GFPL10
Plasmid#98704PurposeCre-dependently express GFPL10 fusion using Introvert SwitchDepositorInsertGFPL10
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationL10: 2609 C->T to remove EcoRI sitePromoterEF1aAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1P-iCLHN
Plasmid#66579PurposeAAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENsDepositorInsertNanoluc-HaloTag
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only