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GST-GFP-ATG14

Plasmid

#171426

Purpose

For the purifcation of GFP- PI3KC3 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ATG14 (ATG14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN iRFP670-NDP52(C443K)

Plasmid

#119695

Purpose

For mammalian expression of iRFP-tagged UBD mutant NDP52

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

iRFP670

Expression

Mammalian

Mutation

C443K

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL1 G116

Plasmid

#123085

Purpose

Gateway entry clone encoding human GABARAPL1 G116

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN iRFP670-OPTN(F178A/D474N)

Plasmid

#119694

Purpose

For mammalian expression of iRFP-tagged LIR/UBD double mutant OPTN

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

OPTN (OPTN Human)

Use

Retroviral

Tags

iRFP670

Expression

Mammalian

Mutation

F178A/D474N

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)

Plasmid

#119692

Purpose

For mammalian expression of LIR double mutant NBR1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

NBR1 (NBR1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

F562A/Y731A & UBD deletion (1-906 ), See depo…

Promoter

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pmStrawberry-Atg4BC74A

Plasmid

#21076

Depositor

Tamotsu Yoshimori

Article

Fujita et al Mol Biol Cell. 2008 Nov . 19(11):4651-9.

Insert

Atg4B(C74A) (Atg4b Mouse)

Use

Tags

mStrawberry

Expression

Mammalian

Mutation

220-222 TGC(Cys) is changed to GCA (Ala)

Promoter

Available since

June 8, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGABARAP

Plasmid

#87871

Purpose

Express a EGFP-human GABARAP in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GABARAP (GABARAP Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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His-ATG3

Plasmid

#202777

Purpose

ATG3 protein (WT) overexpression in E.Coli. 6His-TEVsite-ATG3 WT-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116A

Plasmid

#123104

Purpose

Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116

Plasmid

#123105

Purpose

Expresses 3xFLAG-GABARAPL2 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-GABARAPL1-GFP

Plasmid

#123112

Purpose

Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-GABARAPL1 WT

Plasmid

#123117

Purpose

Expresses EGFP-GABARAPL1 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-GABARAP-GFP

Plasmid

#123111

Purpose

Expresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAP

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Use

Tags

3xFLAG and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-GABARAPL1 G116

Plasmid

#123102

Purpose

Expresses 3xFLAG-GABARAPL1 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-TSF-TEVcs-ULK1K46I

Plasmid

#171417

Purpose

For the purifcation of human ULK1 kinase dead complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

kinase dead

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 G116

Plasmid

#123088

Purpose

Gateway entry clone encoding human GABARAPL2 G116

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-CMV GFP-GABARAPL1 G116A

Plasmid

#123118

Purpose

Expresses EGFP-GABARAPL1 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN GBRP

Plasmid

#89293

Purpose

For expression of the mammalian Atg8 protein GABARAP without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX-IG-ATG3 (H262A)

Plasmid

#212027

Purpose

Expresses untagged ATG3 (H262A) in mammalian cells

Depositor

Michael Lazarou

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 GABARAPL1 no-stop

Plasmid

#123210

Purpose

Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Gateway entry vector / entry clone

Tags

Expression

Mutation

Stop codon removed

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge