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Plasmid#128536PurposeExpresses DsRedDepositorInsertNFAT-DsRed reporter
UseTagsExpressionMammalianMutationPromoterSV40-NFATAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Smad3-shRNA
Plasmid#32806DepositorInsertSmad3 (Smad3 Mouse)
UseRNAiTagsDsRedExpressionMammalianMutationPromoterhU6Available sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJAT10
Plasmid#204293PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDsEndoNA2
Plasmid#174728PurposeExpresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertDsRed-Monomer
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD*RFPEC2
Plasmid#17823DepositorInsertE. coli optimized red flourescent protein (RFPec2)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJAT57
Plasmid#204303PurposeExpresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT11
Plasmid#204290PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT58
Plasmid#204304PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.DepositorInsert3XP3-DsRed
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT71
Plasmid#204305PurposeExpresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXIN2-RFP
Plasmid#99205PurposeRFP expressing bicistronic retroviral vectorDepositorInsertRed fluorescent protein
UseRetroviral; Bicistronic retroviral expression vec…TagsExpressionMammalianMutationThe dsRed cDNA was PCR amplified off AddGene plas…PromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-GFP
Plasmid#106281PurposeA version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.DepositorInsertCMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BFP
Plasmid#106282PurposeA version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.DepositorInsertCMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF.myc.ER-E2-Crimson
Plasmid#38770PurposeIn vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)DepositorInsertE2-Crimson
UseTagsER retention signal (KDEL), ER signal sequence (M…ExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJAT31
Plasmid#204291PurposeFor inserting a phiC31 attP site. HDR integrant is marked with ie1-DsRed, which is expressed in abdomen and mouthparts. Second U6 promoter allows use of two different gRNAs in two synthesized arms.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNHEJ-RPG
Plasmid#85931PurposeConstitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cellsDepositorInsertpPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
UseDual-reporter surrogateTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSSA-RPG
Plasmid#85932PurposeConstitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cellsDepositorInsertpPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
UseDual-reporter surrogateTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
795G
Plasmid#205481PurposeExpresses dsRed and eGFP in both sexes.DepositorInsertDsRed, eGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
795O
Plasmid#205489PurposeFemale specific eGFP with AlutraF, dsRed in both sexesDepositorInsertDsRed, eGFP, AltraF
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only