-
-
pUASp YFP Rab8 CA
Plasmid#37707DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab8 DN
Plasmid#37706DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASP dsRed Rab10 WT
Plasmid#44828DepositorInsertRab10 (Rab10 Fly)
UseDrosophilaTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-NRTN
Plasmid#119896PurposeMouse NRTN In situ hybridization probeDepositorInsertneurturin probe (Nrtn Mouse)
UseIn situ hybridization probeTagsExpressionMutationPromoterAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD401
Plasmid#91828PurposePmyo-2::GFP FP-slot donor for the SapTrap cloning systemDepositorInsertPmyo-2::GFP
UseCRISPRTagsExpressionWormMutationNucleotide 489 of the myo-2 promoter was changed …PromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pERB161
Plasmid#58286Purposeconst. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID)DepositorInsertsMis12-GFP-FKBPx3
inducible FRB-mCh-Mad1^485-584
FKBP-targeted miRNA
UseTagsGFP and mCherryExpressionMammalianMutationamino acids 485-584 of Mad1PromoterAvailable sinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab8 WT
Plasmid#37705DepositorInsertRab8 (Rab8 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceFeb. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design1_mTFP_175_Venus_wt
Plasmid#39828DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pITESceIcmd
Plasmid#218288PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1DepositorInsertHO(-253, -1)-tSynth3UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMMP_kappa
Plasmid#217828PurposeMini-mutagenesis plasmid for kappa light chain YSD FabsDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKESa
Plasmid#211828PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh-EGFR
Plasmid#208281PurposeExpresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh-EGFR
UseLentiviralTagsExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHWBF07EC
Plasmid#130828PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
UseGateway entry vectorTagseGFP-FLAG-HAExpressionMutationPromoterAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pnEA-NpM-Dm4E-T_9-44-Y10AL15A-GB1_AA
Plasmid#148283PurposeBacterial Expression of Dm4E-T_9-44-Y10AL15ADepositorInsertDm4E-T_9-44-Y10AL15A (4E-T Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3(K27Q)
Plasmid#182843Purposea K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
UseTagsGSTExpressionInsectMutationPromoterActin5CAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3
Plasmid#182842PurposeA PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
UseTagsGSTExpressionInsectMutationPromoterActin5CAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bax gRNA#2
Plasmid#171828PurposeCas9-mediated knockout of Bax in mammalian cellsDepositorInsertBax (Bax Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD168 ZF43(RAAR)i
Plasmid#138828PurposeConstitutive expression of ZF43(RAAR)i under the CMV promoter.DepositorInsertZF43(RAAR)i
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43APromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NR2C1_Hormone-recep
Plasmid#109828PurposeProtein expression and purification of NR2C1_Hormone-recepDepositorInsertNR2C1_Hormone-recep (NR2C1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only