EKAR2G_design1_mTFP_175_Venus_wt
(Plasmid
#39828)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 39828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTriEx4
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 7050
-
Vector typeMammalian Expression, Bacterial Expression, Insect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameErk activity reporter
-
SpeciesSynthetic
-
Insert Size (bp)2171
- Promoter CMV
-
Tag
/ Fusion Protein
- His-Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer ctggttattgtgctgtctc
- 3′ sequencing primer tcagtggtatttgtga (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byKarel Svoboda, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
EKAR2G_design1_mTFP_175_Venus_wt was a gift from Olivier Pertz (Addgene plasmid # 39828 ; http://n2t.net/addgene:39828 ; RRID:Addgene_39828) -
For your References section:
A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space. Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O. Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135. 10.1126/scisignal.2004135 PubMed 23882122
Map uploaded by the depositor.