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Plasmid#188499PurposeExpresses FLAG tagged SpdCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CibN-dCas9-CibN
Plasmid#60553PurposeExpresses CibN-dCas9-CibN in mammalian cellsDepositorInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpRY-ABE8e
Plasmid#185671PurposeExpresses SpRY-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-nSpRY
UseCRISPRTagsExpressionMammalianMutationSpRY D10APromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ADAM10-HA
Plasmid#65106PurposeExpression of HA tagged ADAM10 in mammalian cellsDepositorInsertADAM10 (ADAM10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9
Plasmid#46920PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoterDepositorInsertdCas9
UseCRISPRTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP1333
Plasmid#119268Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP
Plasmid#164038PurposeExpression of dCas9-14xGFP11 in mammalian cellsDepositorInsertdCas9-14xGFP11
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and H840A in Cas9PromoterdSV40Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Emi LC
Plasmid#113666PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.DepositorInsertEmicizumab light chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-VP64
Plasmid#50916PurposeTet-regulatable dCas9-VP64 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840A nuclease-deficientPromoterTREAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpRY-HF1-ABE8e
Plasmid#185670PurposeExpresses SpRY-HF1-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-nSpRY-HF1
UseCRISPRTagsExpressionMammalianMutationSpRY-HF1 D10APromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpdCas9 VPR
Plasmid#188509PurposeExpresses FLAG tagged SpdCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max
Plasmid#138155PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRK5-mFzd2
Plasmid#42254DepositorInsertFrizzled 2 (Fzd2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXPR_502_sgCD45
Plasmid#158705PurposeCD45 CRISPRa controlDepositorInsertCD45 (PTPRC Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-VP64 dCas9 VP64-T2A-GFP
Plasmid#59791PurposeCo-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFPDepositorInserthumanized VP64 dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE-VQRCas9
Plasmid#101740Purposeexpression of an optimized Cas9 for base-editing in zebrafishDepositorInsertrAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
UseCRISPRTagsExpressionMammalianMutationD10A, VQR mutationsPromoterCMVAvailable sinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
SadCas9 VPR
Plasmid#188514PurposeExpresses FLAG tagged SadCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/N580APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-OGT1
Plasmid#29760DepositorInsertOGT1 (OGT Human)
UseTagsFlagExpressionMammalianMutationN764D and K1010EPromoterAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-preSci site-CD45)
Plasmid#177889PurposeBaculo expression of GST tag fused to human CD45 ICDDepositorInsertCD45 (aa 598-1304 only) (PTPRC Human)
UseTagsTEV-GST-PreScission cut siteExpressionInsectMutationL652PPromoterPolyhedrinAvailable sinceAug. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
human STIM1 YFP
Plasmid#19754DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsYFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
EE-BE4max
Plasmid#138158PurposeC-to-T base editorDepositorInsertrAPOBEC1(EE)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 R126E + R132E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVhyg
Plasmid#134323PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAHygro(+)LAMP2AS
Plasmid#86146PurposeAnti sense for LAMP2DepositorInsertLAMP2a (LAMP2 Human)
UseTagsExpressionMammalianMutationcontains first 525 bases of LAMP cDNAPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
HiFi dCas9 VPR
Plasmid#188510PurposeExpresses FLAG tagged HiFi dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/R691A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP1104
Plasmid#119248Purposeknockdown pqsC in P. aeruginosaDepositorInsertsgRNA pqsC (P. aeruginosa)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
human STIM1 CFP
Plasmid#19755DepositorInsertSTIM1 (STIM1 Human)
UseRetroviralTagsCFPExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNAHygro(+)LAMP2B
Plasmid#86029PurposeExpresses LAMP2B in Mammalian cellsDepositorInsertLysosome-associated membrane protein 2b (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
HiFi dCas9 KRAB
Plasmid#188500PurposeExpresses FLAG tagged HiFi dCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/R691A/H840APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
YE2-BE4max
Plasmid#138156PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE2)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R132E; within Cas9 D10APromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-mTET1
Plasmid#81052Purposebacterial expression of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
dCBE4-gam
Plasmid#124449PurposeExpresses dead Cas9 (dCas9)-CBE4-gamDepositorInsertdCBE4-gam
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationD10A, H840APromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNICKclos1.0
Plasmid#73639PurposeGenome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824DepositorInsertsCas9 nickase
sGRNA to pyrE
UseE.coli-clostridium shuttle vectorTagsExpressionMutationD10APromoterj23119 and ptbAvailable sinceJune 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trCD45
Plasmid#203753PurposeEncodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrCD45 (PTPRC Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS315e_pGal-dCas9-PmCDA1
Plasmid#79615PurposeExpresses dCas9-PmCDA1 in yeast cellsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionYeastMutationD10A and H840A for nuclease deficient Cas9PromoterpGal1Available sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
SadCas9 KRAB
Plasmid#188504PurposeExpresses FLAG tagged SadCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/N580APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD Mut
Plasmid#72218PurposeExpress flag-tagged mouse Tet1 catalytic domain - catalytic dead mutant.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain (cata…PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
VP64-dCas9
Plasmid#177171PurposeVP64 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertVP64-dCas9(D10A, H840A)
UseCRISPRTagsNLS and NLS-3xFLagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1356
Plasmid#119276Purposefor cloning new sgRNAsDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEL035
Plasmid#137903PurposeSTU2.0 SpyCas9(D10A)-PmCDA1 for plant genome base editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1187
Plasmid#119256Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgNFkB2 guide 2
Plasmid#193594PurposeNFkB2 knockoutDepositorInsertsgNFkB2 guide 2 (NFKB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1189
Plasmid#119257Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9GFP-IRES-Neo
Plasmid#102907PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.DepositorInsertDDdCas9GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KanR neo
Plasmid#167869PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationD10A, D839A, H840A, N863APromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only