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Plasmid#167548PurposeEncodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12DepositorInsertVibrio cholerae O antigens genes of the Inaba serotype
UseCosmidTagsExpressionMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
tol2-CAG::ChR2-YFP
Plasmid#59740PurposeOptogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)DepositorInsertChannelrhodopsin-2
UseTagsYFPExpressionMammalianMutationH134RPromoterCAGAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pY2F
Plasmid#53285PurposeContains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.DepositorInsertlcyE (LUT2 Mustard Weed)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZETAipi
Plasmid#53284PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coliDepositorInsertpds
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458_TEAD1_1
Plasmid#86356PurposeEncodes gRNA for 3' target of human TEAD1DepositorInsertgRNA against TEAD1 (TEAD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HyPer3
Plasmid#119183PurposeAAV expression vector with CMV promoter driving expression of the hydrogen peroxide-sensitive fluorescent biosensor HyPer3.DepositorInsertHyPer3
UseAAVTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamK2-tTA
Plasmid#210731PurposeAAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)DepositorInserttetracycline-controlled transactivator (tTA)
UseAAVTagsExpressionMutationPromoterCamK2Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/intron/GFP
Plasmid#113547PurposeMammalian expression plasmid that can be used with the Flp-In T-REx system.DepositorInsertGFP
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TIGRE-TRE-[poor kozak]Bxb1-ecDHFR-BGHpA
Plasmid#158390PurposeInducible Bxb1 integrase for mammalian cellsDepositorInsertBxb1-ecDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mCherry-GS linker-Zdk1
Plasmid#125226PurposeExpresses mCherry-GS linker-Zdk1 in mammalian cellsDepositorInsertmCherry-GS linker-Zdk1
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
UseTagsExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
R26-pCAG-Ceru-10unit-BGHpA
Plasmid#158387Purpose10-unit intMEMOIR recording array for mammalian cellsDepositorInsertintMEMOIR 10-unit array
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Gg
Plasmid#61653PurposeGateway vector for generating green fluorescent protein fusions in fungiDepositorInsertenhanced green fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
UseTagsExpressionBacterialMutationPromoterlacIqAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-phaZ
Plasmid#169874PurposeSingle guide RNA plasmid for Cas9 targeting phaZ in P. putidaDepositorInsertsgRNA towards phaZ in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-rat-PTP1B
Plasmid#35987DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
UseTagsHis and Xpress Epitope TagExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
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NylCA_pET28a
Plasmid#215418PurposeExpression construct for NylCA gene, codon optimized for expression in E. coliDepositorInsertNylCA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCA-TS_pET28a
Plasmid#215419PurposeExpression construct for NylCA-TS gene, codon optimized for expression in E. coliDepositorInsertNylCA-TS
UseTagsExpressionBacterialMutationS111G/A137L/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2_pET28a
Plasmid#215420PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coliDepositorInsertNylCp2
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NylCp2-TS_pET28a
Plasmid#215421PurposeExpression construct for NylCp2-TS gene, codon optimized for expression in E. coliDepositorInsertNylCp2-TS
UseTagsExpressionBacterialMutationD122G/H130Y/D36A/E263QPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Cg
Plasmid#61652PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE3_IVT
Plasmid#177015PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE3
UseCRISPR; Vector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_NFIA_iso1
Plasmid#104039PurposeDonor vector for 3' FLAG tag of human NFIA_iso1DepositorInsertNFIA_iso1 homology arms (NFIA Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-002
Plasmid#78689PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5
Plasmid#104842Purposeb2AR-SPARK TF componentDepositorInserthLOV1-TEVcs-FLAG-GAL4-V5
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIA_iso1_2
Plasmid#104049PurposeEncodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFPDepositorInsertNFIA_iso1 gRNA (NFIA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIA_iso1_1
Plasmid#104048PurposeEncodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFPDepositorInsertNFIA_iso1 gRNA (NFIA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_NFIL3
Plasmid#86287PurposeDonor vector for 3' FLAG tag of human NFIL3DepositorInsertNFIL3 homology arms (NFIL3 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIL3_2
Plasmid#86349PurposeEncodes gRNA for 3' target of human NFIL3DepositorInsertgRNA against NFIL3 (NFIL3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIL3_1
Plasmid#86348PurposeEncodes gRNA for 3' target of human NFIL3DepositorInsertgRNA against NFIL3 (NFIL3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Plasmid#125233PurposeExpresses "sender" trans-cellular NanoLuc in mammalian cellsDepositorInsertgLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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BC-A1-006
Plasmid#78693PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Yh2
Plasmid#61650PurposeGateway vector for generating yellow fluorescent protein fusions in fungiDepositorInsertenhanced yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-001
Plasmid#78688PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only