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Plasmid#215852PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with CTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTTA
Plasmid#215851PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTA stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTAT
Plasmid#215850PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTAT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TATT
Plasmid#215849PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TATT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-EGFP12-WT(TTTT)
Plasmid#215856PurposeThis plasmid encodes sgRNA that target EGFP with stretch sequenceDepositorInsertsgRNA-EGFP12 with TTTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTTC
Plasmid#215855PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTTC stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TCTT
Plasmid#215853PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TCTT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuperCRISPR-sgRNA-RL69-TTCT
Plasmid#215854PurposeThis plasmid encodes sgRNA that target Renilla luciferase with stretch sequenceDepositorInsertsgRNA-RL69 with TTCT stretch
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-FRB
Plasmid#191327PurposeExpresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
SP_H6_Halo_WT_KDEL_pBABEpu
Plasmid#183688PurposeRetroviral expression of ER targeted H6-tagged Halotag Wild typeDepositorInsertER Halo
UseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FRB-dCer3-CAAX
Plasmid#191326PurposeExpresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-dCer-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mEos3.2
Plasmid#191320PurposeExpresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV lightDepositorInsertTUBB2a-mEos3.2 (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-Dronpa
Plasmid#191321PurposeExpresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV lightDepositorInsertTUBB2a-Dronpa (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c_S(-31)-M30_dT7term
Plasmid#112253PurposeExpresses the S(-31)-M30 apta-FRET RNA origami structure.DepositorInsertS(-31)-M30 apta-FRET RNA origami with T7 promoter and terminators.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28c_S*5-M5_dT7term
Plasmid#112254PurposeExpresses the S*5-M5 apta-FRET RNA origami structure.DepositorInsertS*5-M5 apta-FRET RNA origami with T7 promoter and terminators.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp
Plasmid#179672PurposeMembrane-bound (Lyn) TEVP-inducible caged-cTVMVPDepositorInsertCaged cTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP
Plasmid#179647PurposeHCVP-inducible p450 SEAP RELEASE constructDepositorInsertSEAP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-SEAP-26Sfur-B2AD-SpyTag-tvmvs-KKMP
Plasmid#179648PurposeTVMVP-inducible single TM SEAP RELEASE construct with SpyTagDepositorInsertSEAP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-nTVMVp-AP4-tevs-P3-tevs-cTVMVPmut-CAAX
Plasmid#179662PurposeMembrane-bound (CAAX) TEVP-inducible caged-nTVMVPDepositorInsertCaged-nTVMVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-GFP-ECL-3TM-hcvs-KKMP
Plasmid#179641PurposeHCVP-inducible membrane-bound GFP RELEASE constructDepositorInsertMembrane-bound GFP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMP
Plasmid#179643PurposeOR Gate (TEVP/HCVP) SEAP RELEASE constructDepositorInsertSEAP RELEASE
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/hKGA.S286A
Plasmid#110357PurposeMammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tagDepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTagsV5ExpressionMammalianMutationS286APromotergagAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISK HSUR4
Plasmid#68052Purposeexpresses HSUR4 RNADepositorInsertHSUR4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGD335
Plasmid#219499PurposepAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoterDepositorInsertEco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTII-2A.pMIG II
Plasmid#52112Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsP2A (from porcine teschovirus-1)ExpressionMammalianMutationPromoterAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK013
Plasmid#105345PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK016
Plasmid#105348PurposeBacterial translocation for proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK014
Plasmid#105346PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
BiP 2: C. elegans BiP (hsp-4)-GFP
Plasmid#21896DepositorInsertBiP (hsp-4 Nematode)
UseTagsGFPExpressionWormMutationFusion between the GFP and hsp-4 takes place at A…PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
FRESCA-syntide
Plasmid#132415PurposeFRET-based sensor for CaMKII activityDepositorInsertFRESCA-syntide
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER-RpHLuorin2
Plasmid#171717PurposeLuminal ER expression of ratiometric pHLuorin2DepositorInsertRatiometric pHLuorin2 targeted to ER
UseTagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-GFP/cre
Plasmid#49054PurposeRetroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinaseDepositorInsertGFP/Cre
UseRetroviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRXRα
Plasmid#135400PurposeHuman Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using CMV promoterDepositorInsertHuman retinoid X receptor- alpha
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pBetaActin-TUBB2a-mEos3.2-mEos3.2-mEos3.2
Plasmid#191338PurposeExpresses triple fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light in slice culturesDepositorInsertTUBB2a-mEos3.2-mEos3.2-mEos3.2 (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only