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pHEN2-SG4
Plasmid#196071PurposeExpress wild type SG4 in E.coliDepositorInsertSG4
UseTags3XFLAG and 6XHisExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-gRNA-EF1a-EGFP-CAAX
Plasmid#167936PurposegRNA entry vectorDepositorInsertU6-gRNA backbone
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Neo)
Plasmid#89180PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterTREAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-EGFP-EF1a-rtTA
Plasmid#104454PurposePiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotorDepositorInsertsenhanced green fluorescent protein
reverse tetracycline-controlled transactivator 3
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xHA-miniTurbo-NLS_pCDNA3
Plasmid#107172Purposeexpresses 3xHA-tagged miniTurbo in the mammalian nucleusDepositorInsertminiTurbo (BirA mutant)
UseTagsExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLumi-LgBiT
Plasmid#188319PurposeExpression of LgBiT via sigma 70 promoter in E. coliDepositorInsertLgBiT
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromotersigma 70 promoterAvailable sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMarC9-R6k
Plasmid#89477Purposetransposon plasmid, compatible with Tn-SeqDepositorInsertpMarC9-R6k
UseTagsExpressionMutationPromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL1765 (pSPIN-R, R*MmeI)
Plasmid#160734PurposeSingle-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
moxBFP
Plasmid#68064PurposeC-terminal fusion to oxBFPDepositorTypeEmpty backboneUseTagsmoxBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas9-hHDAC3
Plasmid#98591Purpose3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.DepositorInsertdCAS9-HDAC3 (HDAC3 Synthetic, Human, S. pyogenes)
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1AAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-mCh-Cry2WT
Plasmid#101223PurposeFUS(1-214) fused to mCh-Cry2WTDepositorInsertFUS(aa1-214) (FUS Human)
UseLentiviralTagsmCherry-Cry2ExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGD-p19
Plasmid#196326Purpose35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressorDepositorInserttomato bushy stunt virus p19
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO-sgRNA-puro
Plasmid#104321Purpose3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBeloBac11loxP2272
Plasmid#60342PurposeThis is a BAC vector that contains both wt and mutant loxP sites.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sec61b-V5-TurboID
Plasmid#166971PurposeExpresses ER targeted TurboID for secretome biotinylationDepositorInsertSec61b-V5-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEE401E
Plasmid#71287PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 enhancer fused to EC1.1 promoter and U6-26p…Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-cytoEKAR4
Plasmid#174438PurposeCytosol-targeted EKAR4.DepositorInsertcytoEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-E-cadherin-RFP
Plasmid#79603PurposepHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging systemDepositorInsertCDH1 promoter region (CDH1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX_305_Ovalbumin
Plasmid#184924PurposeLentiviral vector for expressing Ovalbumin. Also expresses the puromycin resistance gene as a selectable marker.DepositorInsertOvalbumin (OVAL Chicken)
UseLentiviralTagsExpressionMammalianMutationOur vector has an A188T mutation that does not im…PromoterPGKAvailable sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-pcDNA3
Plasmid#18950DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pU6-Cj-sgRNA
Plasmid#89753PurposeU6 promoter driven expression of Cj-SgRNA cloned with BsmbIDepositorInsertCj-SgRNA
UseSgrna expression under u6 promoterTagsExpressionMutationPromoterU6Available sinceMay 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3C(1-10)
Plasmid#117482PurposeLentiviral vector for constitutive expression of sfCherry3C(1-10)DepositorInsertsfCherry3C(1-10)
UseLentiviralTagsExpressionMammalianMutation5 substitutions (K45R, G52D, T106A, K182R, N194D)…PromoterSFFVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPP-mCherry-hTRIM21
Plasmid#104972PurposeLentiviral Transfer Plasmid with mCherry-tagged human TRIM21DepositorInsertTripartite Motif containing 21 (TRIM21 Human)
UseLentiviralTagsmCherryExpressionMutationPromoterSFFVAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Pa1
Plasmid#138475PurposeEnables high-efficiency recombineering in P. aeruginosaDepositorInsertsPapRecT
mutL E36K
UseTagsExpressionBacterialMutationE36KPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Renilla luciferase
Plasmid#118016PurposeConstitutive expression of Renilla luciferaseDepositorInsertRenilla luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-DsRed
Plasmid#64703PurposeVector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsertpiggyBac-3xP3-DsRed
UseTagsExpressionMutationPromoter3xP3Available sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.1
Plasmid#111053PurposeExpresses dLight1.1 in mammalian cellsDepositorInsertdLight1.1
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY3
Plasmid#158198PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only
