Addgene

We narrowed to 4,919 results for: pcDNA3.1+

Showing: 4361 - 4380 of 4919 results

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pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R

Plasmid

#166806

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

P1214R

Promoter

CMV

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 AURKA deltaNter

Plasmid

#157749

Purpose

Expression of AURKA without Nter in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

Expression

Mammalian

Mutation

the first 30AA of Aurora A are lacking

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 mitoAURKA

Plasmid

#157750

Purpose

Expression of AURKA targeted to mitochondria

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

Expression

Mammalian

Mutation

Aurora A without the first 30AA + MTS of CoxVIII

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-OLIG2-Adel-IDR

Plasmid

#145249

Purpose

Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

OLIG2-Adel-IDR (OLIG2 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 AIDx no UGI

Plasmid

#135349

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S2 1058 APOBEC3 no UGI

Plasmid

#135350

Purpose

Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-rAPOBEC3-ID-1058

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP APOBEC3 no UGI

Plasmid

#135352

Purpose

Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

rAPOBEC3-nSpyCas9 aa 1029

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 AIDx with UGI

Plasmid

#135354

Purpose

Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

hAIDx-nSpyCas9 aa-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 2

Plasmid

#135358

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

WT Tada-nSpyCas9 aa 1029-evolved Tada

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 CP ABEMax strategy 3

Plasmid

#135359

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 CP nSpyCas9 ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 miniABEMax V82G strategy1

Plasmid

#135360

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

mini ABEMax 1029 nSpyCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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S1 1029 V82G ABEMax Strategy 3

Plasmid

#135363

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

1029 V82G ABEMax

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD11wt-IDR

Plasmid

#145242

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD11wt-IDR (HOXD11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD11-Adel-IDR

Plasmid

#145241

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD11-Adel-IDR (HOXD11 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13+7A-IDR

Plasmid

#145239

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13+7A-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-44A-IDR

Plasmid

#145237

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-44A-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-15A-IDR

Plasmid

#145236

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-15A-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HEY2wt-IDR

Plasmid

#145235

Purpose

Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HEY2wt-IDR (HEY2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-GSX2wt-IDR

Plasmid

#145233

Purpose

Expresses fusion of GAL4 DNA-binding domain and GSX2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

GSX2wt-IDR (GSX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EOMES-Adel-IDR

Plasmid

#145230

Purpose

Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

EOMES-Adel-IDR (EOMES Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Showing: 4361 - 4380 of 4919 results

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