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Plasmid#216548PurposeFor expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
V96 ELP
Plasmid#68392PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residueDepositorInsertV96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK7-HA-S6K1-WT
Plasmid#8984DepositorInsertS6K1 (Rps6kb1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG CLTX-CAR
Plasmid#157743Purposeknock CLTX-CAR into AAVS1 safe harbor locus for constitutive expressionDepositorInsertchlorotoxin containing chimeric antigen receptor targeting glioblastoma
UseCRISPR, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB045a
Plasmid#79224PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
GHRHR-Tango
Plasmid#66292PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGHRHR (GHRHR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-SaCas9
Plasmid#129553PurposeContains codon-optimized SaCas9 and gRNA for streptomycesDepositorInsertSaCas9
UseTagsExpressionBacterialMutationPromoterrpsL(XC)-BbsIAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF3169
Plasmid#144645PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGTF2I (GTF2I Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDM1047
Plasmid#108990PurposeInducible expression vector (dox-on), N-terminal GFP taggingDepositorTypeEmpty backboneUseDictyostelium discoideum inducible expression vec…TagsGFPExpressionMutationPromoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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TFORF1919
Plasmid#141887PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hGli2 flag3x
Plasmid#84920Purposemammalian expression of Gli2DepositorInsertGli2 (GLI2 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB UbC Arrestin-uTEV2-V5; UAS-mCherry
Plasmid#173123PurposeExpresses uTEV2 and reporter receiver construct in DIPGDepositorInsertArrestin-uTEV2-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-WT
Plasmid#59365PurposePlasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInserthuman NAT10 (NAT10 Human)
UseTagsFLAGExpressionMammalianMutationMutations to render cDNA resistant to a siRNAPromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyn-tailed mCherry-SEpHluorin
Plasmid#32002DepositorInsertMembrane-targeting sequence of Lyn kinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF0112
Plasmid#141519PurposeLentiviral vector for overexpressing the SMAD2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMAD2 (SMAD2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-V5-Topo mCherry
Plasmid#128062PurposeLentiviral vector for C terminal mCherry fusionsDepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163029PurposeFLiCRE TF for neuronal expressionDepositorInsertNrxn3b-MKII-hLOV1-TEVcs-tTA
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hSOX2
Plasmid#20724DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMSP3545
Plasmid#46888PurposeGram-Positive Bacterial Shuttle Vector for Nisin-Controlled Inducible ExpressionDepositorTypeEmpty backboneUseNisin-inducible expression; gram-positive bacteri…TagsExpressionBacterialMutationPromoterPnisAAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMT416
Plasmid#8607DepositorInsertbarnase, barstar
UseTagsphoA signal sequence to barnaseExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-GPR151
Plasmid#190133Purposeexpresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cellsDepositorInserteGFP-p2A-HA-GPR151
UseLentiviralTagsHA, eGFP, and p2AExpressionMammalianMutationPromoterCMVAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKW0776
Plasmid#119024PurposeE.coli expression plasmid for PmSPS2DepositorInsertPmSPS2
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-fLuc=tdT
Plasmid#120870PurposePiggybac transposon plasmid for live animal optical imagingDepositorInsertpcDNA3-fLuc=tdT
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-APEX2-NIK3x
Plasmid#129274PurposeExpression of APEX2 in the nucleolusDepositorInsertGFP-APEX2-NIK3x
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
LPH2781 - Ub-K63R
Plasmid#32160DepositorUseTagsExpressionYeastMutationK63RPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAIDFA-EF1a-NmScarlet-mAID
Plasmid#140615PurposeExpression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1 mutated F74A
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME-mCherry-NS
Plasmid#108885PurposeMultisite gateway vector for 5' tagging with mCherryDepositorInsertmCherry
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5
Plasmid#60532PurposeDonor vector for human Pak5DepositorInsertPak5 (PAK5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000338399
Plasmid#78156PurposeshXPO1 targeting CDSDepositorInsertXPO1 (XPO1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRL_SRSF2_P95H_mCherry
Plasmid#84023Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95HPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp160
Plasmid#100926PurposeMammalian expression plasmid for Env from the DU422.1 HIV-1 isolateDepositorInsertHIV-1 (DU422.1) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H187
Plasmid#170348PurposemT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR2-FusionRed
Plasmid#105669PurposeExpresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoterDepositorHas ServiceAAV Retrograde and AAV1InsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMVMagFRET-1-NLS
Plasmid#50748PurposeExpresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.DepositorInsertgene encoding for MagFRET-1 with NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Kan
Plasmid#105233PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescensDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe bleo human PPAR gamma 2
Plasmid#11439DepositorInsertPPAR gamma 2 (PPARG Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-Ef1a-TVA-2A-N2cG
Plasmid#172360PurposeTargeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-PROGERIN
Plasmid#140189PurposeOverexpression of Progerin with a GFP fusion protein, under human synapsin promotorDepositorInsertProgerin (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
Plasmid#65418PurposeAAV packaging vector for CamKIIa driven KOR DREADD expressionDepositorInsertCaMKIIa-HA-KORD-IRES-mCitrine (OPRK1 Human)
UseAAVTagsHA and mCitrineExpressionMutationPromoterCaMKIIaAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Mito-HyPerGRt
Plasmid#173198PurposeAAV transfer plasmid for a HyPer7 and tdTomato fusion under hSyn promoterDepositorInsertHyper7-tdTomato
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
NPY5R-Tango
Plasmid#66456PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertNPY5R (NPY5R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nanog5P reporter
Plasmid#16337DepositorInsertNanog promoter (Nanog Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterMouse Nanog promoterAvailable sinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-VP64-BFP
Plasmid#46912PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFPDepositorInsertsdCas9-VP64-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags3xNLS, BFP, and VP64 domainExpressionMammalianMutationPromoterLTR and PGKAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMK347 (BSD-P2A-mAID)
Plasmid#121181PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro
Plasmid#183410PurposepiggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cellsDepositorInsertKRAB-dCas9-ecDHFR
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc-Neo-OVA
Plasmid#22533DepositorInsertOVA (OVAL Chicken)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGb_PMLlxP_EGFP
Plasmid#140283Purposea targeting plasmid for EGFP-PMLDepositorInsertEGFP
UseMouse TargetingTagsExpressionMutationPromoterendogenousAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only