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Showing: 8721 - 8760 of 148587 results

  5. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  6. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Use
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  10. IDG_CACNB2_OE_1

    Plasmid
    #161684
    Purpose
    Overexpresses CACNB2
    Depositor
    Mike McManus
    Insert
    CACNB2 (CACNB2 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    161684-plasmid-map-sequence-id-319671

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  15. EF1a_mCherry_P2A_Hygro

    Plasmid
    #135003
    Purpose
    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135003-plasmid-map-sequence-id-272070

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  16. pDONR221-SLC8A3_STOP

    Plasmid
    #161352
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC8A3 (SLC8A3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161352-plasmid-map-sequence-id-355047

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  17. pDONR221-SLC8A2_STOP

    Plasmid
    #161340
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC8A2 (SLC8A2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161340-plasmid-map-sequence-id-355046

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  18. TFORF0405

    Plasmid
    #143212
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CTCFL (CTCFL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143212-plasmid-map-sequence-id-396092

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  19. pCW57.1 KBTBD4 WT

    Plasmid
    #184624
    Purpose
    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cells
    Depositor
    Vincenzo D'Angiolella
    Article
    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1
    Insert
    N-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    no mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184624-plasmid-map-sequence-id-363386

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  20. TFORF0757

    Plasmid
    #143074
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NOTCH1 (NOTCH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143074-plasmid-map-sequence-id-399050

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  26. pCSN067

    Plasmid
    #101748
    Purpose
    Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and ā€¦
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101748-plasmid-map-sequence-id-199897

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  30. INCENP

    Plasmid
    #39185
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    INCENP (INCENP Human)
    Use
    Tags
    GST-Tev
    Expression
    Bacterial
    Mutation
    Complex with AURKBA-c004
    Promoter
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

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  36. pPP085

    Plasmid
    #158795
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasĪ¦-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158795-plasmid-map-sequence-id-309510

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Showing: 8721 - 8760 of 148587 results