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VSVG-YFP-2xUVR8

Plasmid

#49800

Purpose

expresses VSVG fused to UVR8 for light-triggered trafficking

Depositor

Matthew Kennedy

Article

Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

Insert

VSVG-YFP-2xUVR8

Use

Tags

HA, MYC, and YFP

Expression

Mammalian

Mutation

VSVG contains ts045 mutations

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sizzled

Plasmid

#24684

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
pBS mNotch1-IC (DM#270)

Plasmid

#15079

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

Notch1-IC (Notch1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

K1750A

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2

Plasmid

#111086

Purpose

For production of recombinant siRNAs (pro-siRNAs) in E. coli.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Type

Empty backbone

Use

RNAi

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCYP29

Plasmid

#212862

Purpose

Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 2

Plasmid

#30160

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al Neural Dev. 2008 . 3():15.

Insert

APLP2 shRNA 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pET29b-Ava3858-3856

Plasmid

#45745

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3856

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pCFD4d

Plasmid

#83954

Purpose

Modified from pCFD4, with the vermillion marker and the attB site removed

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

dU6-1; dU6-3

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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SA-betageo

Plasmid

#21709

Depositor

Philippe Soriano

Article

Friedrich et al Genes Dev. 1991 Sep . 5(9):1513-23.

Insert

SAbgeo

Use

Mouse gene trapping

Tags

Expression

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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VSVG-mEOS2-2xUVR8

Plasmid

#49803

Purpose

expresses VSVG fused to UVR8 for light-triggered trafficking

Depositor

Matthew Kennedy

Article

Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

Insert

VSVG-mEOS-2xUVR8

Use

Tags

HA, MYC, and mEOS2

Expression

Mammalian

Mutation

VSVG contains ts045 mutations

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b-Ava3858-3855

Plasmid

#45746

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3855

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pKSII/INSeGFP

Plasmid

#53787

Purpose

1 kb zebrafish insulin promoter driving eGFP

Depositor

Larry Moss

Article

diIorio et al Dev Biol. 2002 Apr 1;244(1):75-84.

Insert

INS promoter/eGFP/pA

Use

Zebrafish

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

zebrafish insulin/ZIP

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only
pAG306-GPD-empty chr I

Plasmid

#41895

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sizzled HA

Plasmid

#24683

Depositor

Edward De Robertis

Article

Lee et al Cell. 2006 Jan 13. 124(1):147-59.

Insert

Sizzled (szl Frog)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2010

Availability

Academic Institutions and Nonprofits only
pCYP01

Plasmid

#212834

Purpose

Expresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCB1532H-RFP

Plasmid

#101855

Purpose

Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

Depositor

Sophien Kamoun

Article

Pennington et al Figshare (2017)

Type

Empty backbone

Use

Fungal expression plasmid, tested in magnaporthe

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLRD12

Plasmid

#208264

Purpose

Production of miltiradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cldB
smKAL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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UVR8-GFPCaaX

Plasmid

#49805

Purpose

expresses UVR8 fused to GFP with a prenylation motif for membrane targeting

Depositor

Matthew Kennedy

Article

Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

Insert

UVR8-GFPCaax (UVR8 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pACT4-DM

Plasmid

#111233

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Article

Worseck et al Methods Mol Biol. 2012;812:63-87. doi: 1

Insert

ccdB-cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge