Addgene

Showing: 4341 - 4360 of 54090 results

Results per page:

Sort results by:

pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Human, Rat, Mouse, Bovine)

Use

Tags

Nt: histag-3C protease site

Expression

Bacterial

Mutation

Promoter

T7 polymerase

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
DMPK

Plasmid

#155550

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DMPK (DMPK Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CNOT3

Plasmid

#155467

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CNOT3 (CNOT3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

Plasmid

#166693

Purpose

Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

Depositor

Elvir Becirovic

Article

Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

Inserts

dCas9-VPR
3x Cnga1 promoter-targeting sgRNAs

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

TRE and U6

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SERPINH1

Plasmid

#155999

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SERPINH1 (SERPINH1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-neo

Plasmid

#166467

Purpose

Empty vector to express overexpression cDNA

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PLX_307 FDX1

Plasmid

#184495

Purpose

Lentivirus expression for FDX1

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-2xFLAG-2xSTREP (empty vector)

Plasmid

#172604

Purpose

Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Puro

Plasmid

#170317

Purpose

Expresses HA and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

Plasmid

#178851

Purpose

Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)

Use

Tags

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L/L254A

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR v2 puro hGSDME gRNA2

Plasmid

#223520

Purpose

Knocking out hGSDME in human cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR v2 puro hGSDME gRNA1

Plasmid

#223519

Purpose

knocking out hGSDME in human cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
plentiCRISPv2_FDX1-2

Plasmid

#184489

Purpose

Lentivirus all in one CRISPR vector targeting human FDX1 gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-TIR1-HA

Plasmid

#172610

Purpose

Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

TIR1

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Tet/ON Minimal CMV promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2-TetR-neoR

Plasmid

#153310

Purpose

Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Insert

TetR

Use

Transposon

Tags

Expression

Mutation

Promoter

SV40

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p2K7bsdUBI-mCherry-STIM1

Plasmid

#114178

Purpose

Lentiviral vector for expression of mCherry-STIM1

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Lentiviral

Tags

mCherry (inserted after signal peptide)

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR-HA-IKKbeta

Plasmid

#15470

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase beta (Ikbkb Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2007

Availability

Academic Institutions and Nonprofits only
ADARB1

Plasmid

#155396

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ADARB1 (ADARB1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ZNF9

Plasmid

#156152

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ZNF9 (CNBP Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge