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Plasmid#110101PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phagesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
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pGL3 Promoter Vector
Plasmid#212939PurposeControl vector with constitutive firefly luciferase expressionDepositorInsertluc+
UseLuciferaseTagsExpressionMutationWTPromoterSV40Available sinceJan. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTDH3-dCas9
Plasmid#46920PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoterDepositorInsertdCas9
UseCRISPRTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
C1-F-tractin-mCherry
Plasmid#155218PurposeMonitor global F-actin distributionDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseTagsExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Murine E-cadherin mCherry
Plasmid#71366Purposemammalian expression of murine E-caderin with mCherry fusionDepositorInsertE-cadherin (Cdh1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKL09-GCaMP6f-mScarlet bb118
Plasmid#158979PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.DepositorInsertGCaMP6f-mScarlet fusion
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23118 (sigma 70)Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A
Plasmid#103865PurposeCatalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).DepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationContains the H133A and H1058A mutations which ina…PromoterEF1aAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLOX-gfp-iresTK
Plasmid#12243DepositorInsertGFP, HSV1-TK
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RH
Plasmid#60516PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
UseTags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ube1/PET21d
Plasmid#34965DepositorInsertUbe1 (UBA1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLDLR-Luc (aka: pES7)
Plasmid#14940DepositorInsertLDLR promoter (LDLR Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceMay 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
hE-cadherin-pcDNA3
Plasmid#45769DepositorInserthE-cadherin (CDH1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEPIP_Peptide_Cloning_Vector
Plasmid#172110PurposeLentiviral plasmid for protein / peptide overexpressionDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEf1-alphaAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABE-zeo largeTgenomic
Plasmid#1778DepositorInsertlarge T and small T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEIGW roGFP2-Orp1
Plasmid#64993PurposeMammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pRN3P_membrane_EGFP
Plasmid#139402Purposein vitro transcription for mRNADepositorInsertmembrane-EGFP
UseIn vitro transcriptionTagsEGFPExpressionMutationPromoterT3Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-tdTomato-tDeg
Plasmid#217521PurposeExpresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoterDepositorInserttdTomato-tDeg fluorogenic protein
UseTagsExpressionMammalianMutationPromoterminiCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-Rex
Plasmid#122134PurposeB. subtilis Rex sensor plasmid with gentamicin resistance and pBBR1 originDepositorInsertB. subtilis Rex
UseTagsExpressionBacterialMutationPromoterapFAB338Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-C1
Plasmid#58186DepositorTypeEmpty backboneUseTagsSNAP tagExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDrVSP-IRES2-EGFP
Plasmid#80333PurposeExpresses Danio rerio VSP with separated EGFP in mammalian cellDepositorInsertDaino rerio voltage-sensing phosphatase (tpte Zebrafish)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-hVAP-A
Plasmid#104447PurposeExpression of human VAP-A fused to GFP in mammalian cellsDepositorInsertvesicle-associated membrane protein-associated protein A (VAPA Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-IFT20-EGFP
Plasmid#118032PurposeExpresses IFT20-EGFP in mammalian cellsDepositorInserthuman IFT20 (IFT20 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Sport6-CD9-pHluorin
Plasmid#130905PurposeExpression of CD9-pHluorin for visualization of multivesicular body-plasma membrane fusion.DepositorInsertCD9-pHluorin (CD9 Human, Aequorea victoria)
UseTagspHluorinExpressionMammalianMutationphluorin inserted between Asn-50 and Asn-51 in ex…PromoterCMVAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -