-
Plasmid#21075PurposeMammalian expression of rat LC3 fused to mRFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsmRFPExpressionMammalianMutationFirst ATG is not included.PromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_HaloTag7_T2A_EGFP
Plasmid#169325PurposeExpression of HaloTag7 in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-ZmBbm-P2A-ZmWus2-9
Plasmid#197750PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmBbm-P2A-ZmWus2 bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GIPR-DuET
Plasmid#213252PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGIPR (GIPR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CVB3 IRES
Plasmid#189968PurposePart 2 plasmid to introduce the CVB3 IRES into circRNAsDepositorInsertCVB3 IRES
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-AsCas12a
Plasmid#113430PurposeExpresses 10xHis-MBP fused to AsCas12aDepositorInsertAsCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIB1
Plasmid#28245PurposeExpresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activationDepositorUseTagsCIB1ExpressionYeastMutationPromoterADH1Available sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SSAP-MS2-BB_BbsI
Plasmid#183826PurposepU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFPDepositorInsertU6-guideRNA-dCas9-T2A-EBFP
UseCRISPRTagsExpressionMammalianMutationPromoterCBHAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorInsertWilms tumor 1 associated protein (WTAP Human)
UseTagsFLAG tagExpressionMammalianMutationPromoterT7Available sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pCALSL-mir30
Plasmid#13786DepositorInsertmir30 cassette
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXPR_071
Plasmid#164558PurposeHigher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.DepositorInsertsgRNA cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-LaminB
Plasmid#98830PurposeIn vivo visualization of the nuclear envelope (can be used for colocalization studies)DepositorArticleInsertLaminB
UseTagsmTurquoise2ExpressionMammalianMutation*(see below)PromoterCMVAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
YFP-Parkin-IRES-zeo
Plasmid#61728PurposeExpression of human Parkin fused to eYFPDepositorInsertParkin (PRKN Human)
UseTagseYFPExpressionMammalianMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mPiezo1-IRES-eGFP
Plasmid#80925Purposeexpresses mouse Piezo1 with GFPDepositorInsertmouse Piezo1 CDS (Piezo1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pHR_Gal4UAS_pGK_mCherry
Plasmid#79124PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
UseTagsExpressionMutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_WT
Plasmid#98651PurposeExpresses MBP-tagged full length FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1
Plasmid#184395PurposeDonor plasmid for stable integration of firefly luciferase control reporter at AAVS1DepositorInsertFirefly luciferase beta-globin
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only