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Plasmid#153212PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-CD63-(TEV)-tTA
Plasmid#162597Purposehuman CD63-TEV cleavage site-tTADepositorInserthuman CD63 (CD63 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM38869
Plasmid#153220PurposeLevel 0 Arabidopsis U6-promoter vectorDepositorInsertArabidopsis U6 snRNA promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b-2AeGFP
Plasmid#121945PurposeMammalian expression, Genome editingDepositorInsertAaCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL90003
Plasmid#68262PurposeLevel 0 Golden Gate Part: Promoter U6 (Triticum aestivum)DepositorInsertPromoter: U6 (Triticum aestivum)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry
Plasmid#206219PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1
Plasmid#110623PurposeIt expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.DepositorInsertPH mScarlet-I- IRES- sYFP2 PH
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNTD
Plasmid#157869Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domainDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaNTDPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Gh
Plasmid#61648PurposeGateway vector for generating green fluorescent protein fusions in fungiDepositorInsertenhanced green fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-Rab7A Q86L
Plasmid#28050DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and 3xFlagExpressionMammalianMutationQ86LPromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLC242-GFP-Sestrin2
Plasmid#100519PurposeExpresses GFP tagged Sestrin2 in mammalian cells to make lentivirusDepositorInsertSESN2 (SESN2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh2.1 GFP
Plasmid#209100PurposeGFP expressing scramble of shRNA targeting Cdh2DepositorInsertscrCdh2.1
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-H29A
Plasmid#80602PurposeExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-H29A
UseAAVTagsExpressionMammalianMutationHis29 mutated to AlaPromoterCBAAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-S-Luc_cyto
Plasmid#113926PurposePlasmid encoding the cytosolic localization of the chimeric SNAP-NanoLuc fusion S-Luc in mammalian cellsDepositorInsertS-Luc
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_H-Luc
Plasmid#113924PurposePlasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-LucDepositorInsertH-Luc
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CuGFP-ATG8(416)
Plasmid#49423PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseTagsGFPExpressionBacterial and YeastMutationPromoterCUP1Available sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cry2(1-531)-mCh-BAXS184E
Plasmid#117238PurposeLight activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCry2531-mCh-BAXS184E
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shSOD2
Plasmid#102976PurposeSuppression of SOD2DepositorInsertshSOD2 (SOD2 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PAmCherry1-PH_PLCd1
Plasmid#92167PurposePI(4,5)P2 single molecule fluorescent reporterDepositorInsertiRFP-PAmCherry1-PH_PLCd1
UseTagsiRFP-PAmCherry1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-mKdm4d(H189A)-polyA
Plasmid#61554PurposeFor mRNA synthesis or expression in mammalian cellsDepositorInsertKdm4d (catalytic mutant)
UseTagsflagExpressionMammalianMutationchanged Histidine 189 (of Kdm4d) to AlaninePromoterCMV and T7Available sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only