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Showing: 10801 - 10850 of 148131 results

  2. FRP2370

    Plasmid
    #127576
    Purpose
    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1
    Insert
    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    127576-plasmid-map-sequence-id-252228

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  4. pCDNA3 HA C9ORF72 op

    Plasmid
    #74156
    Purpose
    Express HA tagged optimized C9ORF72 in mammalian cells
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    C9ORF72 (C9orf72 Human, Synthetic)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    optimized sequence
    Promoter
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    74156-plasmid-map-sequence-id-139165

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  8. hprtSAGFP

    Plasmid
    #41594
    Purpose
    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences
    Depositor
    Maria Jasin
    Article
    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.
    Insert
    SA-GFP reporter
    Use
    Mouse Targeting; Dsb repair reporter substrate
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2012
    Availability
    Academic Institutions and Nonprofits only
    41594-plasmid-map-sequence-id-228244

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  17. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Yubin Zhou
    Article
    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1
    Insert
    LOV2 and STIM1 cytosolic fragment
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    101245-plasmid-map-sequence-id-209272

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  19. TFORF2026

    Plasmid
    #144326
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NPAS3 (NPAS3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144326-plasmid-map-sequence-id-404179

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  20. TFORF1958

    Plasmid
    #144118
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CUX1 (CUX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144118-plasmid-map-sequence-id-396192

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  23. LPUtopia-7

    Plasmid
    #199212
    Purpose
    Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Inserts
    aminoglycoside phosphotransferase,
    enhanced Green Fluorescence Protein
    Red Fluorescence Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and thymidine kinase promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199212-plasmid-map-sequence-id-396462

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  30. pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site

    Plasmid
    #172508
    Purpose
    Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172508-plasmid-map-sequence-id-344931

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  31. pU6_(GLuc)_INT[3xMS2_SL]

    Plasmid
    #68426
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three MS2 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68426-plasmid-map-sequence-id-125556

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  35. mCherry-CenpA-N-22

    Plasmid
    #62742
    Purpose
    Localization: Nucleus, N1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    CenpA (CENPA Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pAP84

    Plasmid
    #86554
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Depositor
    Wiep Klaas Smits
    Insert
    Pveg-sLucopt
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86554-plasmid-map-sequence-id-173277

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  50. pEF-BFP

    Plasmid
    #138272
    Purpose
    Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555
    Insert
    BFP H66 PAM
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H66, PAM motif
    Promoter
    Ef1a
    Available since
    March 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    138272-plasmid-map-sequence-id-273617

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Showing: 10801 - 10850 of 148131 results