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PurposeTMPRSS2 with N-terminal HA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154963 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepCSDest
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Modifications to backboneAdded N-terminal HA tag, Tag added by Q5-site directed mutagenesis
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHA-TMPRSS2
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SpeciesH. sapiens (human)
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GenBank IDNM_005656.4
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Entrez GeneTMPRSS2 (a.k.a. PRSS10)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byhttps://www.addgene.org/53887/
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSDest-HA-TMPRSS2 was a gift from Utpal Pajvani (Addgene plasmid # 154963 ; http://n2t.net/addgene:154963 ; RRID:Addgene_154963) -
For your References section:
Angiotensin converting enzyme 2 is a novel target of the gamma-secretase complex. Bartolome A, Liang J, Wang P, Ho DD, Pajvani UB. Sci Rep. 2021 May 7;11(1):9803. doi: 10.1038/s41598-021-89379-x. 10.1038/s41598-021-89379-x PubMed 33963249