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Plasmid#203848PurposeExpression of GCaMP7f in CaMKII expressing cellsDepositorInsertjGCaMP7f
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FUS/TLS-FLAGC
Plasmid#60362PurposePlasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.DepositorInsertFUS (FUS Human)
UseTagsFLAG and GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
plenti hSyn GAPDH
Plasmid#220918PurposeNeuronal specific expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-D4
Plasmid#155363PurposeExpresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertNb H11-D4
UseTagsHis6ExpressionMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP rbFOX2
Plasmid#63086PurposeMammalian expression of EGFP tagged FOX2DepositorInsertRBFOX2 (RBFOX2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_18xTEAD_luc2
Plasmid#173003Purposefirefly luciferase reporter vector with 18x clustered TEAD binding sitesDepositorInsert18xTEAD-luc2
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgRNA(F+E)-sgGal4
Plasmid#100549PurposesgGal4-expressing vector modified from Addgene plasmid #51024DepositorInsertsgGal4
UseCRISPRTagssgRNAExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT3T4
Plasmid#50595PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-26t plus, OsU3p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)
Plasmid#209077PurposeNgn2 mediated cortical neuron induction, dox induction is includedDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-c-Met
Plasmid#31786DepositorInsertMet (MET Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-3xrGBD
Plasmid#176100PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSExpressionMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pABE8e-protein
Plasmid#161788PurposeRhamnose-inducible expression of 8xHIS-ABE8e in E. coliDepositorInsertABE8e base editor
UseTags8xHIS and BPNLSExpressionBacterialMutationPromoterpRhaBADAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pS34.EF1α<GFP-2A-Cas9-RC
Plasmid#207404PurposeCas9-RC and GFP from an EF1α promoter. High-performance knockin through DSB repair by homology-directed recombination (HDR) or homology-mediated end joining (HMEJ). [Lab plasmid ID: N213]DepositorInsertCas9-RC
UseCRISPR and Synthetic BiologyTagsGFP-2AExpressionMammalianMutationPromoterEF1αAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52
Plasmid#98678PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase IIDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF2161
Plasmid#141950PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGRHL1 (GRHL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV06.2c_DART-VADAR_iRFP720-sensor
Plasmid#200410PurposepDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2DepositorInsertClosed-loop DART VADAR sensor detecting iRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Neo_CAG-Cas9
Plasmid#86698PurposeCas9 expression vector for targeting to the AAVS1 locusDepositorInsertCas9
UseCRISPRTagsExpressionMammalianMutationPromoterpCagAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
c-Myc-5-HT2A
Plasmid#67944PurposeN-terminally c-myc tagged human 5-hydroxytryptamine receptor 2ADepositorInsertHTR2A (HTR2A Human)
UseTagsc-MycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On shYAP1-9
Plasmid#193668PurposeTet inducible knockdown of YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe_Hyg_cOVA_T2A_mStrawberry
Plasmid#161737PurposeExpression of cOVA and mStrawberryDepositorInsertcytoplasmic ovalbumin
UseRetroviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pcDNA3
Plasmid#109486Purposemammalian expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
Plasmid#67974PurposeCRISPR gRNA expression vector with an improved scaffold and puro/BFP markersDepositorInsertU6gRNA cassette, PGKpuro2ABFP cassette, WPRE
UseLentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSmo
Plasmid#25395DepositorInsertmouse Smoothened cDNA (Smo Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP
Plasmid#176282PurposeViral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertTeNT-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only