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Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3ā¦PromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B mCherry-XL (R125K)
Plasmid#189926PurposeExpresses nuclear-localized mCherry-XL (R125K) in mammalian cellsDepositorInsertmCherry-XL (R125K)
UseTagsH2BExpressionMammalianMutationdiffers from mCherry by R125K, W143S, I161V, Q163ā¦PromoterCMV7Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lck-mTurquoise2
Plasmid#98822PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorArticleInsertLck
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-Halotag-FRB-EX-nes_pLX304
Plasmid#120913PurposeLentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosolDepositorInsertHA-Halotag-FRB-EX-NES
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgRNA_expression_vector
Plasmid#210212PurposeAn empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.DepositorTypeEmpty backboneUseTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 neo
Plasmid#98292PurposeVariant of lentiCRISPRv2 that confers G418 resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-PP7-2xmCherry-CAAX
Plasmid#74925PurposeExpresses PP7-2xmCherry-CAAXDepositorInsertPP7-2xmCherry-CAAX
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMito-rxRFP1.1
Plasmid#67841Purposeredox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localizedDepositorInsertrxRFP1.1 and mitochondrial localization sequence
UseTagsHis Tag and mitochondrial localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-GFP
Plasmid#185473PurposeMammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PIP2
Plasmid#60937PurposeFluorescent FPX sensor for PIP2DepositorInsertPIP2 dimerization dependent sensor RA+B monomers
UseTagsdimerization dependent fluorescent protein monomeā¦ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
Plasmid#71396Purpose2Gi2R assayDepositorInserts2xGFP:NES
2xRFP:NLS
UseLentiviralTagsIRESExpressionMammalianMutationPromoterEF1alpha and IRESAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGPA1
Plasmid#115476PurposeGeneration of mariner-based transposon mutant libraries in Enterococcus faeciumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_sfCherry2(1-10)
Plasmid#82602PurposeExpresses sfCherry2(1-10) fusion in mammalian cellsDepositorInsertsfCherry2(1-10)
UseTagsExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
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mRFP-Rab5
Plasmid#14437PurposeMammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.DepositorInsertRab 5 (RAB5A Human)
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRZ
Plasmid#27663DepositorTypeEmpty backboneUseRna transcriptionTagsHDV RibozymeExpressionMutationPromoterAvailable sinceApril 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOpen-RNAse H
Plasmid#165575PurposeEndonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.DepositorInsertRNAse H
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits