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rat APOBEC1-EGFP
Plasmid#112857Purposeplasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion proteinDepositorInsertapolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD-IFP2.0C
Plasmid#215750PurposeExpresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP-IFP2.0C
Plasmid#215752PurposeExpresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xCBX7CD-IFP2.0C
Plasmid#215754PurposeExpresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensorsDepositorInsert2xCBX7CD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC12
Plasmid#205068PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 12 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC16
Plasmid#205069PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 16 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC18
Plasmid#205070PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 18 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC20
Plasmid#205071PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 20 amino acidsPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTF1 deltaC9
Plasmid#205067PurposeMTF1 protein expressionDepositorInsertMTF1 (MTF1 Budding Yeast)
UseTagsExpressionBacterialMutationLacks C-terminal 9 amino acidsPromoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo W42A
Plasmid#191399PurposeW42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo W42A
UseTags3xFLAGExpressionMammalianMutationW42APromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
OKSIM
Plasmid#24603DepositorUseCre/Lox and LentiviralTagsExpressionMammalianMutationOct4-2A-KLF4-2A-Sox2-IRES-MycPromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
tdTurboRFP-Farnesyl-5
Plasmid#58057PurposeLocalization: Plasma Membrane, Excitation: 553, Emission: 576DepositorInsertFarnesyl (HRAS Human)
UseTagstdTurboRFPExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cwc25-pRSETA
Plasmid#61043PurposeEncodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.DepositorInsertCwc25
UseTagsTEV site, Histidine TagExpressionBacterialMutationPromoterT7Available sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA0057
Plasmid#131784PurposeGuide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.DepositorInsert20mer GFP cassette guide (gGFP) and 5' sgRNA
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_C9orf72:1-481
Plasmid#210987PurposeMammalian expression of full-length human C9orf72. C-terminal HaloTag.DepositorInsertC9orf72:M1-F481 (C9orf72 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_DDB1:1-1140
Plasmid#211007PurposeMammalian expression of full-length human DDB1. C-terminal HaloTag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_PAF1:1-531
Plasmid#211076PurposeMammalian expression of full-length human PAF1. C-terminal HaloTag.DepositorInsertPAF1:M1-D531 (PAF1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_RAB7A:1-207
Plasmid#211096PurposeMammalian expression of full-length human RAB7A. C-terminal HaloTag.DepositorInsertRAB7A:M1-C207 (RAB7A Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNICBio2_WDR5:2-334
Plasmid#211027Purposebacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.DepositorInsertWDR5:A2-C334 (WDR5 Human)
UseTagsMHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHEExpressionBacterialMutationwild typePromoterT7Available sinceApril 4, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
