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Plasmid#73358PurposeIn vivo protein protein interaction studies/ expression in mammalian cellsDepositorInsertTMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMV immediate early promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T E7 del PTLHE
Plasmid#13637DepositorInsertHPV 16 E7 del PTLHE (E7 Human)
UseTagsGSTExpressionBacterialMutationdel aa 6-10PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pComb3XSS
Plasmid#63890Purpose3rd generation plasmid for phage display on modified geneIII, contains stuffer fragmentDepositorInsertsSS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer
UsePhage displayTags6x His, HA tag, and geneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonor MCS Rosa26
Plasmid#37200DepositorInsertsRosa26 left targeting arm
MCS
Rosa26 right targeting arm
UseGenomic targeting - zinc fingerTagsExpressionMutationPromoterAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-GCaMP6s
Plasmid#105714PurposeCan be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.DepositorHas ServiceAAV8InsertGCaMP6s
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hOCT4
Plasmid#20726DepositorInsertOCT4 (POU5F1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-TARG1
Plasmid#172594PurposeFor purification (from E.coli) of His-tagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2FA CO Tol2 TPase
Plasmid#133032PurposeExpression vector for production of codon optimized Tol2 transposaseDepositorInsertCodon optimized Tol2 Transposase
UseIn vitro expressionTagsExpressionMutationCodon OptimizedPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p102_gRNA_Sa_mClover
Plasmid#213778PurposeCloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.DepositorInsertSa gRNA
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTargeT gE
Plasmid#60845PurposeExpression of the varicella zoster glycoprotein (gE) in mammalian cellsDepositorInsertORF68 (ORF68 )
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2
Plasmid#174665PurposeExpresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoterDepositorInsertsLUC2
mCherry:T2A:Hygro
UseLentiviral and LuciferaseTagsmCherry and hygromycin dual reporter gene linked …ExpressionMammalianMutationPromoterHuman cytomegalovirus immediate early enhancer/pr…Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL0_20 [panARS-opt ORI]
Plasmid#198933PurposeLevel 0 partDepositorInsertpanARS-opt ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hMYC
Plasmid#20723DepositorInsertc-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEC3135 (pERASE)
Plasmid#220306PurposeEnables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO3
Plasmid#24318DepositorInsertAGO3 (AGO3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Plasmid#20298PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsEYFPExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only