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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUW
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Backbone manufacturerCarlos Lois
- Backbone size w/o insert (bp) 8382
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsTOP10, 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOCT4
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Alt namePOU5f1
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SpeciesH. sapiens (human)
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Insert Size (bp)1083
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LNCX
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUW-tetO-hOCT4 was a gift from Rudolf Jaenisch (Addgene plasmid # 20726 ; http://n2t.net/addgene:20726 ; RRID:Addgene_20726) -
For your References section:
A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Hockemeyer D, Soldner F, Cook EG, Gao Q, Mitalipova M, Jaenisch R. Cell Stem Cell. 2008 Sep 11. 3(3):346-53. 10.1016/j.stem.2008.08.014 PubMed 18786421