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Showing: 10751 - 10800 of 148108 results

  8. MK1283

    Plasmid
    #71428
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709
    Depositor
    Howard Salis
    Article
    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …
    Promoter
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71428-plasmid-map-sequence-id-440608

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  9. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  12. rAAV2/cMG.WPP

    Plasmid
    #184542
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-cMG.WPP capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-cMG.WPP capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R; insertion of WPPKT…
    Promoter
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184542-plasmid-map-sequence-id-361933

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  23. TFORF0260

    Plasmid
    #142499
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF4 (TCF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142499-plasmid-map-sequence-id-397239

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  25. pMax_ScaI_BFP

    Plasmid
    #177824
    Purpose
    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).
    Depositor
    Zachary Nagel, Leona Samson
    Article
    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5' inserted ScaI RE cut site
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177824-plasmid-map-sequence-id-348950

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  28. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. Epac-S-H187

    Plasmid
    #170348
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170348-plasmid-map-sequence-id-336137

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  41. SARS-CoV2 Nucleocapsid

    Plasmid
    #153201
    Purpose
    Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells
    Depositor
    Peter Klein
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11
    Insert
    Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Use
    Tags
    Myc tag (5X)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 15, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153201-plasmid-map-sequence-id-305776

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  43. pLV CMV FST-FLAG

    Plasmid
    #175167
    Purpose
    Lentiviral expression of human FST-FLAG
    Depositor
    Larry Gerace
    Insert
    FST (FST Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    175167-plasmid-map-sequence-id-352509

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  47. rAAV2/cMG.QRP

    Plasmid
    #184543
    Purpose
    AAV packaging plasmid, expressing Rep2 and AAV-cMG.QRP capsid
    Depositor
    Minmin Luo
    Article
    Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s
    Insert
    AAV-cMG.QRP capsid
    Use
    AAV; Helper vector for packaging
    Tags
    Expression
    Mutation
    changes on AAV9 capsid: K449R; insertion of QRPPR…
    Promoter
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184543-plasmid-map-sequence-id-361939

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  49. TFORF0257

    Plasmid
    #141560
    Purpose
    Lentiviral vector for overexpressing the TCF4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF4 (TCF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141560-plasmid-map-sequence-id-276816

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Showing: 10751 - 10800 of 148108 results