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pIBA2-Int-SpyTag
Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
UseTagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO-AKT1
Plasmid#170682Purposedoxycycline-inducible overexpression of AKT1DepositorInsertAKT1 (AKT1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Anti-TASK1 K+ channel [N374/48R]
Plasmid#188160PurposeMammalian Expression Plasmid of anti-TASK1 K+ channel (Rat). Derived from hybridoma N374/48DepositorInsertanti-TASK1 K+ channel (Rattus norvegicus) recombinant mouse monoclonal antibody (Kcnk3 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GPR82-Tango
Plasmid#66375PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR82 (GPR82 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
gGBEv6.3-SpG
Plasmid#202630Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpG(D10A)-MPGv6.3
UseTagsExpressionMammalianMutationSpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…PromoterhU6, EF1a, CBhAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP SNAP47
Plasmid#101915PurposeExpresses SNAP47 in mammalian cellsDepositorInsertSNAP47 (SNAP47 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hfYFP
Plasmid#186523PurposeHyperfolder YFP fluorescent protein: cytosolic expression in mammalian cellsDepositorInsertHyperfolder YFP
UseTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A&C-BEmax
Plasmid#157947PurposeMammalian expression of A&C-BEmaxDepositorInsertA&C-BEmax
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0675
Plasmid#141659PurposeLentiviral vector for overexpressing the PRDM16 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPRDM16 (PRDM16 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN-FKBP DD(L106P)-UbK0G75S-sfGFP
Plasmid#170157PurposeExpression for LIBRON constructsDepositorInsertFKBP DD (L106P)-UbK0G75S
UseRetroviralTagssfGFPExpressionMutationchange all lysin residues of ubiquitin to Arg, G7…PromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV.hSyn.GLPLight-ctr
Plasmid#187468PurposeExpresses GLPLight-ctr in mammalian cellsDepositorInsertGLPLight-ctr
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTET2 CD mutant
Plasmid#89896PurposeExpresses mouse TET2 CD mutant in mammalian cellsDepositorInsertTET2 (Tet2 Mouse)
UseTagsFlagExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA
Plasmid#205318PurposeAll-in-one AAV vector expressing ISDge10 TnpB and its reRNADepositorInserthumanized ISDge10 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-APOBEC1-YTHmut
Plasmid#131637PurposeExpresses APOBEC1 fused to a mutant YTH domain with diminished m6A bindingDepositorInsertAPOBEC1-YTHmut-HA (YTHDF2 Rat, Human)
UseTagsHAExpressionMammalianMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterCMVAvailable sinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3
Plasmid#189755PurposeProduces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet-I3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro
Plasmid#141386PurposeLentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-M (M SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGM1190
Plasmid#69994PurposeOriT containing mobilizable vector for thiostrepton induced gene expression in StreptomycesDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF160_VSV-G
Plasmid#225963PurposeCMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.DepositorInsertCMV-Intron-VSVG (env protein)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM
Plasmid#164783PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
UseTagsGLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GeNL/pcDNA3
Plasmid#85200PurposeGreen color variant of bright luminescent proteinDepositorInsertGreen enhanced Nano-lantern
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorInsertNES-PhoCl-mCherry
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME Actin-Vhh sfGFP (JDW 1215)
Plasmid#224495PurposeGateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)DepositorInsertActin-Vhh-sfGFP
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-All-in-one-hCascade
Plasmid#134919PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.DepositorInsertCascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cFos-tTA-pA
Plasmid#66794PurposeExpresses TetActivator protein under the control of the c-fos promoter for use in TetTagingDepositorInsertcfos promoter linked to the tet activator gene
UseAAVTagsnoExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
12XCSL-DsRedExpressDL
Plasmid#47683PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsdsRedExpressDRExpressionMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TCF21
Plasmid#192894PurposeBarcoded piggybac transposon vector with Dox-inducible expression of TCF21DepositorInsertTCF21 (TCF21 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)
Plasmid#129027Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
UseTagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO2A1_STOP
Plasmid#161115PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO2A1 (SLCO2A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)
Plasmid#197511PurposeCbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacerDepositorInsertAAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
UseAAV and CRISPRTagsBPNLS and BPNLS-NpuC(intein)ExpressionMutationC-terminal mutations of SpRY(L1111R/D1135L/S1136W…PromoterCbhAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-Ecad
Plasmid#205188PurposeThis vector encodes of a synCAM (Ecad) and GFPDepositorInsertsynCAM Ecad and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM33
Plasmid#65398PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM33 (TRIM33 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-E
Plasmid#78852PurposeIntroduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsCas9-P2A-PuroExpressionMammalianMutationPromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP
Plasmid#85427PurposeLentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expressionDepositorInserthuman IL-10
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLck-mVenus-C1
Plasmid#84337PurposeExpresses a mVenus tagged with a membrane localization sequenceDepositorInsertLck (MGCVCSSNPELPVAT)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
UseTagsHis tagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mMaroon1
Plasmid#83840PurposeExpresses mMaroon1DepositorInsertmMaroon1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-NP-V5-B/BRISBANE/60/2008
Plasmid#187923PurposeExpresses C terminal V5 tagged nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cellsDepositorInsertInfluenza B Nucleoprotein
UseTagsV5ExpressionMammalianMutationPromoterCMV PromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p18sheLL
Plasmid#37321DepositorInsertHPV18L1+L2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
dSpCas9-VPR
Plasmid#205247PurposeIn vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.DepositorInsertdSpCas9-VPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsPABPC1_I
Plasmid#146542PurposeMammalian Expression of HsPABPC1DepositorInsertHsPABPC1 (PABPC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GAK-pmCherryN1
Plasmid#27695DepositorInsertGAK (Gak Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-SynTetOff-GFP
Plasmid#190231PurposeAAV vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGE32
Plasmid#63159PurposeExpress sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplastDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 promoter for gRNA/PTG expression a…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-BRCA1-mCherry
Plasmid#195275Purposeexpress mCherry-BRCA1 fusionDepositorInsertmCherry-BRCA1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only
