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Plasmid#132956PurposecrRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombeDepositorInsertdFnCas12a
UseCRISPR and Synthetic BiologyTagsSV40 NLS and nucleoplasmin NLSExpressionMutationPromoteradh1Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Guide-Generating Vector
Plasmid#125752PurposeGuide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assemblyDepositorInsertCys4-GFPdropout-SpCas9scaffold
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB8622
Plasmid#126912PurposePlasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiaeDepositorInsertADE2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
jk089_h12
Plasmid#112151PurposeC. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)DepositorInsertC. thermophilum DASH/Dam1 complex
UseTagshis tag on Spc34 and strep tag on Dad1ExpressionMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc-Cdc3-Cdc10
Plasmid#131160PurposeExpression of Septin rodsDepositorInsertCdc3 + Cdc10
UseBicistronicTagsCdc10: S TagExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYCTK009
Plasmid#176713PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Eremothecium cymbalariae (sender part).DepositorArticleInsertmfalpha1Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK020
Plasmid#176724PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Eremothecium cymbalariae (receiver part).DepositorArticleInsertste2Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK030
Plasmid#176734PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Candida albicans (barrier part).DepositorArticleInsertbar1Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK031
Plasmid#176735PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Eremothecium cymbalariae (barrier part).DepositorArticleInsertbar1Ec
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK008
Plasmid#176712PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Candida albicans (sender part).DepositorArticleInsertmfalpha1Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDH6
Plasmid#83777PurposeUseful for tagging yeast proteins with C-terminal YFP tag; G418 selectionDepositorInsertYFP
UseTagsYFPExpressionYeastMutationS65G, V68L, Q69K, S72A, T203Y mutations added.PromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS313-Sad1-Wt
Plasmid#90308Purposeyeast expressionDepositorInsertSAD1
UseTagsno tagExpressionYeastMutationPromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYCTK034
Plasmid#176738PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).DepositorArticleInsertbar1Sc
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p184.3
Plasmid#44521DepositorInsertHistone 3 N-terminus
UseTagsGSTExpressionBacterialMutationaa1-46PromoterAvailable sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDZ264 (pKAN tdtomato cyc loxP KAN loxP)
Plasmid#35193DepositorInserttdtomato cyc loxP KAN loxP
UseTagsExpressionMutationPromoterAvailable sinceMarch 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
YIp204-P30-His-POL30
Plasmid#99550PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 markerDepositorInsertPOL30 (POL30 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterPOL30Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Esf1
Plasmid#99987PurposeExpress yeast Esf1 with Y2H binding domain fusionDepositorInsertEsf1 (ESF1 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMBD-Gate1
Plasmid#25946DepositorInsert
UseTagsGAL4 Fusion ProteinExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
LHP308 - Ub - wt
Plasmid#32151DepositorUseTagsExpressionYeastMutationPromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-Scs2
Plasmid#58834PurposeExpresses GFP-Tagged Scs2 in yeastDepositorInsertScs2 (SCS2 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only