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Plasmid#71633PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Val/Phe79>Trp mutant (TdPurE2-S38V,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to valine, phenylalanine-79 to …PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK659
Plasmid#71634PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Cys/Phe79>Trp mutant (TdPurE2-S38C,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to cysteine, phenylalanine-79 t…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK660
Plasmid#71635PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Met/Phe79>Trp mutant (TdPurE2-K41M,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to methionine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK661
Plasmid#71636PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Lys41>Arg/Phe79>Trp mutant (TdPurE2-K41R,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges lysine-41 to arginine, phenylalanine-79 t…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK662
Plasmid#71637PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp/Cys87>Ser mutant (TdPurE2-F79W,C87S)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges phenylalanine-79 to tryptophan, cysteine-…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK689
Plasmid#71642PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Ser38>Asn/Phe79>Trp mutant (TdPurE2-S38N,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges serine-38 to asparagine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK697
Plasmid#71646PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met15>Trp/Ser38>Thr mutant (TdPurE2-M15W,S38T)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges methionine-15 to tryptophan, serine-38 to…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK657
Plasmid#71647PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Ala/Phe79>Trp mutant (TdPurE2-H40A,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges histidine-40 to alanine, phenylalanine-79…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK658
Plasmid#71648PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, His40>Asn/Phe79>Trp mutant (TdPurE2-H40N,F79W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges histidine-40 to asparagine, phenylalanine…PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK505
Plasmid#71594PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe mutant (AaPurE1fww)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-34 to phenylalanine, additiona…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK506
Plasmid#71596PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp165>Phe mutant (AaPurE1wwf)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationchanges tryptophan-165 to phenylalanine, addition…PromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACVA
Plasmid#29421DepositorInsertAmber-Cerulean-Venus-Amber
UseStoichiometry standardTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
VCAA
Plasmid#29422DepositorInsertVenus-5-Cerulean-5-Amber-6-Amber
UseStoichiometry standardTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
ACAV
Plasmid#29420DepositorInsertAmber-Cerulean-Amber-Venus
UseStoichiometry standardTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
R777-E164 Hs.PIK3CA-nostop
Plasmid#70448PurposeGateway ORF clone of human PIK3CA [NM_006218.2] without stop codon (for C-terminal fusions)DepositorInsertPIK3CA (PIK3CA Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
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R777-E036 Hs.DUSP2-nostop
Plasmid#70320PurposeGateway ORF clone of human DUSP2 [NM_004418.3] without stop codon (for C-terminal fusions)DepositorInsertDUSP2 (DUSP2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
H2B-RFP in pENTR1A (w507-1)
Plasmid#22525DepositorInsertHuman Histone H2B -Heteractis crispa red fluorescent protein (H2BC21 Human)
UseEntry vectorTagsHcRedExpressionMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
PGR-Blue
Plasmid#68374PurposeTerminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.DepositorTypeEmpty backboneUseSynthetic Biology; Golden gate assemblyTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only