Addgene

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Showing: 4281 - 4300 of 16227 results

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pAG551-MAP4-greenFAST

Plasmid

#160366

Purpose

Expresses MAP4-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-SH2-KDEL

Plasmid

#209716

Purpose

Mammalian expression of SH2 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-SH2-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo

Plasmid

#153422

Purpose

MSCV with IRES-Neomycin resistant gene

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAG372-mito-greenFAST

Plasmid

#160357

Purpose

Expresses mito-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

mito-greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

Plasmid

#48296

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-2A-TAL6-MSTN

Plasmid

#72597

Purpose

MSTN TALEN with 2A-GFP tagged

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

TALEN for MSTN

Use

TALEN

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Ago4

Plasmid

#21536

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago4 (AGO4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pME-FlEx switch

Plasmid

#82406

Purpose

Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-10nmSAH-GFP(1-10)

Plasmid

#61660

Purpose

10nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-10nmSAH-GFP(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFM689

Plasmid

#74635

Purpose

alias pFA6a-mCherry-hphNT1 - vector for C-terminal tagging of yeast genes with mCherry and hygromycin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Inserts

Use

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 E76A

Plasmid

#8321

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

E76A (activated mutant)

Promoter

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-cjBLIMP1-Venus-HygTK

Plasmid

#141028

Purpose

Reporter integration

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Tags

Expression

Mutation

Promoter

CMV-SP6

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-eArch3.0-mRuby2-ST

Plasmid

#109135

Purpose

Archaerhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eArch3.0-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PAmKate

Plasmid

#32691

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Use

Tags

Expression

Bacterial

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

araB

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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FCK-QuasAr2-mOrange2 eFRET

Plasmid

#59173

Purpose

Fluorescent membrane voltage sensor

Depositor

Adam Cohen

Article

Zou et al Nat Commun. 2014 Aug 13;5:4625. doi: 10.

Insert

QuasAr2-mOrange2 eFRET

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pIM690

Plasmid

#74638

Purpose

alias pFA6a-yTagRFP-T-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and hygromycin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Use

Tags

Expression

Yeast

Mutation

Promoter

none

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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AlphaC

Plasmid

#124525

Purpose

pColE1_AlphaC

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBP-TL5

Plasmid

#72989

Purpose

RBS_M2

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M2 RBS

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only