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Plasmid#145576PurposeBacterial Expression plasmid for SARS-CoV-2 endoRNAseDepositorInsertSARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046826
Plasmid#145687PurposeBacterial Expression plasmid for SARS-CoV-2 nsp8DepositorInsertSARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MYMH 391
Plasmid#138059PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23101- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 392
Plasmid#138053PurposeEncodes Anderson promoter variant using mRFP as a reporter geneDepositorInsertJ23102- pSEVA-331Bb- RFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 539
Plasmid#138078PurposeControl vector with no degradation tag to compare against various degradation tag variantsDepositorInsertJ23104-mCherry::sbfI- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH41
Plasmid#138035PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-7GT-GFP
UseTagsExpressionBacterialMutationAAA GAG TAG AAAPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 553
Plasmid#138083PurposeDegradation tag variant at C-terminal of mCherryDepositorInsertJ23104-mCherry-NYADAS-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 552
Plasmid#138082PurposeDegradation tag variant at C-terminal of mCherryDepositorInsertJ23104-mCherry-DAS- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 551
Plasmid#138081PurposeDegradation tag variant at C-terminal of mCherryDepositorInsertJ23104-mCherry-AAV- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 550
Plasmid#138080PurposeDegradation tag variant at C-terminal of mCherryDepositorInsertJ23104-mCherry-LVA- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 549
Plasmid#138079PurposeDegradation tag variant at C-terminal of mCherryDepositorInsertJ23104-mCherry-LAA- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 473
Plasmid#138077PurposeSynthetic Terminator L3S1P47 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-L3S1P47-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 472
Plasmid#138076PurposeSynthetic Terminator L3S2P44 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-L3S2P44-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 471
Plasmid#138075PurposeSynthetic Terminator L3S2P24 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-L3S2P24-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 470
Plasmid#138074PurposeSynthetic Terminator L3S3P21 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-L3S3P21-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 469
Plasmid#138073PurposeSynthetic Terminator L3S2P21 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-L3S2P21-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 468
Plasmid#138072PurposeNatural Terminator BB0010 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-BB0010-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 467
Plasmid#138071PurposeNatural Terminator ECK120051401 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-51401-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 466
Plasmid#138070PurposeNatural Terminator ECK120010799 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-10799-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 465
Plasmid#138069PurposeNatural Terminator ECK120010818 inserted between mCherry and GFPDepositorInsertJ23104-mCherry-10818-GFP-pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only