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pMOD3-mNeptune2-Sh_ble

Plasmid

#120336

Purpose

Bacterial mNeptune2 promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Use

Tags

Expression

Bacterial

Mutation

Promoter

(None)

Available since

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-K560-24xGCN4_v4

Plasmid

#72229

Purpose

A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4 SunTag peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

K560 kinesin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pACYC-Taq

Plasmid

#102793

Purpose

Expresses Taq DNA polymerase under WT T7 promoter

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Taq DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2G_2

Plasmid

#118225

Purpose

Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_2

Plasmid

#118226

Purpose

Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKFSS1_2

Plasmid

#118227

Purpose

Empty E. coli - B. burgdorferi shuttle vector; streptomycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-mito-mCherry-24xGCN4_v1

Plasmid

#60913

Purpose

A fusion protein of the SunTag 24xGCN4 v1 peptide array to a mitochondrial targeting domain and mCherry

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

mito-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-sfGFP

Plasmid

#61058

Purpose

A direct fusion of sfGFP to the 24xGCN4_v4 peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v4 peptide array and sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCFD6

Plasmid

#73915

Purpose

tissue-specific multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

:tRNA:gRNA:tRNA:gRNA:tRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAS-DSCP

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS338

Plasmid

#73719

Purpose

SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP by CRISPR/Cas9

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Unc-32::GFP(Cbr-unc-119)

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-NPM WT

Plasmid

#17578

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pOPH6

Plasmid

#40315

Depositor

Shaun Lott

Article

Loo et al Protein Expr Purif. 2002 Feb;24(1):90-8.

Insert

Peptide:N-glycosidase F

Use

Tags

HexaHistidine and OmpA3 leader

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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PAP-delta10-cHIS

Plasmid

#21716

Depositor

Andrew Bohm

Article

Balbo et al Biochemistry. 2005 May 31. 44(21):7777-8

Insert

polyA polymerase (PAP1 Budding Yeast)

Use

Tags

HIS

Expression

Bacterial

Mutation

30 amino acids off the c terminus

Promoter

Available since

Aug. 22, 2009

Availability

Academic Institutions and Nonprofits only
mito-EcSTH

Plasmid

#211921

Purpose

Mitochondrial localization of EcSTH

Depositor

Binghui Li

Article

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

mito-EcSTH

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/Lux-nat

Plasmid

#120324

Purpose

Bacterial luminescence

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pSAM

Plasmid

#45174

Depositor

Jumi Shin

Article

Sathiamoorthy et al PLoS One. 2012;7(10):e47259. doi: 10.137

Type

Empty backbone

Use

Tags

6xHis and 6xHis/Thrombin/T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
pMOD3-mClover-nat

Plasmid

#120328

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Use

Tags

Expression

Bacterial

Mutation

D103N (see comments below)

Promoter

(None)

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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p156

Plasmid

#186179

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 156

Use

Synthetic Biology and Unspecified; Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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