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Plasmid#99906PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPIK16045_pX330_Flag-SuperFi-Cas9
Plasmid#184370PurposeMammalian expression of SuperFi-Cas9 nucleaseDepositorInsertSuperFi-Cas9
UseCRISPRTags3X FLAG and NLSExpressionMammalianMutationY1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…PromoterCBhAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab11WT
Plasmid#46785Purposeexpression of WT Rab11a in mammalian cellsDepositorInsertRAB11A (RAB11A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Ch
Plasmid#61647PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mc2 puro
Plasmid#206215PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL90002
Plasmid#68261PurposeLevel 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)DepositorInsertPromoter: U6-26 (Arabidopsis thaliana)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1 G224A-mGFP
Plasmid#180819PurposeExpression of human PD-1 G224A fused to mGFPDepositorInsertPD-1 G224A (PDCD1 Human)
UseLentiviralTagsExpressionMutationPD-1 (G224A)PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast EF1a
Plasmid#206223PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPNL1
Plasmid#61651PurposeGateway vector for generating nucleus-targeted fluorescent protein fusions in fungiDepositorInsertmonomeric red fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p53 R175H
Plasmid#39480DepositorInsertTP53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationP72R and R175HPromoterAvailable sinceSept. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-miR9/9*
Plasmid#177805PurposeThe pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.DepositorInsertmiR-9/9*
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBT-peRNA_GG-Puro
Plasmid#173220PurposepiggyBac transposon containing hU6 promotor and pegRNA cloning siteDepositorInsertspegRNA cloning cassette
PuroR
UsePiggybacTagsExpressionMammalianMutationPromoterEF1-a and hU6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15α1
Plasmid#101712PurposeGoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPRCΔ15Ω1
Plasmid#101710PurposeGoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR lociDepositorTypeEmpty backboneUseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltalinker
Plasmid#157870Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the linkerDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltalinerPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh20.1 GFP
Plasmid#209106PurposeGFP expressing scramble of shRNA targeting Cdh20DepositorInsertscrCdh20.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
JH03
Plasmid#73512PurposemKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2DepositorInsertpmex-5::mKate2::PH::tbb-2
UseTagsmKate2ExpressionMutationPromotermex-5Available sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGM1202
Plasmid#69615Purposeinducible gene expression in Streptomyces, C-terminal His-tag encoding sequenceDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only