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Plasmid#52985Purposesplit Venus attached to MCP and PCP respectivelyDepositorInsertnls-ha-MCP-VenusN-nls-ha-PCP-VenusC
UseLentiviralTagsExpressionMutationPromoterubcAvailable sinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTubb3-MC
Plasmid#87112Purposeminicircle parental backbone plasmid including GFP knock-in donor targeting Tubb3 gene and one cutting site for HITIDepositorInsertGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔPS-ΔgeneIII
Plasmid#134351PurposeHelper phage for M13 phage productionDepositorInsertHelper phage gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)
Plasmid#188380PurposeLentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet30b-5M SrtA
Plasmid#51140Purposeexpression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activityDepositorInsertS.aureus SrtA 5M
UseTags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available sinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJYDNp
Plasmid#162451PurposePositive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.DepositorInsertAga2p-eUnaG2
UseShuttle vector bacteria/yeastTagsHA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2
Plasmid#191566PurposeExpresses an shRNA and mTagBFP2DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 ZoAl-ENT N-1F (R1103A)
Plasmid#216548PurposeFor expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0513
Plasmid#141625PurposeLentiviral vector for overexpressing the PAX5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX5 (PAX5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
Plasmid#172508PurposeEncodes the mature DisCas7-11 DR along with a golden gate site for spacer cloningDepositorInsertpU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hSOX2
Plasmid#20724DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pC1.509
Plasmid#203955PurposeLevel 1 self-replicating vector containing a DAPG inducible FnCas12aDepositorInsertPphlF-Cpf1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lyn-tailed mCherry-SEpHluorin
Plasmid#32002DepositorInsertMembrane-targeting sequence of Lyn kinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
hGli2 flag3x
Plasmid#84920Purposemammalian expression of Gli2DepositorInsertGli2 (GLI2 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-PDK1-FLAG-LOV*
Plasmid#202205PurposeExpresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cellsDepositorInsert[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)
UseTagsFLAG and LOV*ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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NeonGreen
Plasmid#197924PurposeMonomer expression vectorDepositorInsertNeonGreen
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Kan
Plasmid#105233PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescensDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#82400PurposeGateway-compatible zebrafish elavl3 promoterDepositorInsertelavl3 promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.LSL.EGFP
Plasmid#100047PurposeAAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a DIO-rsChRmine-oScarlet-Kv2.1-WPRE
Plasmid#183529PurposeOptogeneticsDepositorInsertrsChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationI146M/G174SPromoterEf1aAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xEcoLeuT(CUA)_AnapRS
Plasmid#140019PurposePlasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of azido-phenylalanine; for transient or stable piggyBac-mediated integrationDepositorInsertEcoLeuRS (AnapRS)
UseTagsExpressionMammalianMutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…PromoterEF1Available sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
ECFP-H2B-6
Plasmid#55344PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorInsertH2B (H2BC11 Human)
UseTagsECFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-N1_SEPT2
Plasmid#71547Purposemammalian expression of Sept 2 with a GFP fusionDepositorInsertSeptin 2 (Septin2 Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PDHA1-N-10
Plasmid#55118PurposeLocalization: Mitochondria, Excitation: 587, Emission: 610DepositorInsertPDHA1 (PDHA1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOpen-T4 PNK
Plasmid#165520PurposeThe most commonly used polynucleotide kinase.DepositorInsertT4 PNK
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti6-V5-Topo mCherry
Plasmid#128062PurposeLentiviral vector for C terminal mCherry fusionsDepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK7-HA-S6K1-WT
Plasmid#8984DepositorInsertS6K1 (Rps6kb1 Rat)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
PX404 Campylobacter jejuni Cas9
Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorInsertCjCas9
UseCRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR2-FusionRed
Plasmid#105669PurposeExpresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoterDepositorHas ServiceAAV Retrograde and AAV1InsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianMutationPromoterCaMKIIaAvailable sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE046-SiT-Cas12a-[Repr]
Plasmid#128132PurposeExpress both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Repr]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-RN
Plasmid#60503PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSYFP2-C1
Plasmid#22878DepositorInsertSYFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL3 1kb prom. CD274
Plasmid#107002PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR
Plasmid#112159PurposeGABA SensorDepositorHas ServiceAAV1InsertiGABASnFR
UseAAVTagsExpressionMutationPromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-PROGERIN
Plasmid#140189PurposeOverexpression of Progerin with a GFP fusion protein, under human synapsin promotorDepositorInsertProgerin (LMNA Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-APEX2-NIK3x
Plasmid#129274PurposeExpression of APEX2 in the nucleolusDepositorInsertGFP-APEX2-NIK3x
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJC005.gent
Plasmid#182739PurposeCRISPR-Cas12a genome editing of E. faeciumDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQO18-TEV
Plasmid#27399DepositorInsertleuT
UseTagsHisX10 and TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBJ FLAG-hPar1
Plasmid#53226Purposemammalian expression of human Par1 with N-terminal FLAG tagDepositorInsertPar1 (F2R Human)
UseTagsFLAG and bovine prolactin signal sequence (MDSKGS…ExpressionMammalianMutationDeletion of aa1-39 (native signal peptide)PromoterSV40Available sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Dvl2-GFP
Plasmid#109148PurposeStable Dvl2 expressionDepositorInsertDvl2 (DVL2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterSV40Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
ClpP-his
Plasmid#22144DepositorInsertClpP-His6
UseTagshisExpressionBacterialMutationC-terminal His tagPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
p5E-4xnrUAS (L4-R1)
Plasmid#61372Purpose5' Gateway Entry Clone Containing 4xnrUASDepositorInsert4xnrUAS
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF0256
Plasmid#142496PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTCF4 (TCF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAIDFA-EF1a-NmScarlet-mAID
Plasmid#140615PurposeExpression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1 mutated F74A
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG469-LifeAct-redFAST
Plasmid#160365PurposeExpresses LifeAct-redFAST in mammalian cellsDepositorInsertLifeAct-RedFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only