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Plasmid#49154Purposeexpression of Sec61 beta fused to BFP, used as a general ER markerDepositorInsertSec61 beta (SEC61B Human)
UseTagsBFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-Strep-tagII-kanMX6
Plasmid#20757DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionMutationPromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
Gq-CASE
Plasmid#168125PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F124/E125 within GNAQPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NFIA+SOX9
Plasmid#129455PurposeInducible overexpression of NFIA and Sox9 in human cells.DepositorInsertSox 9 (SOX9 Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KMT2A_MLLT10
Plasmid#205843PurposeExpress mEGFP-tagged fusion protein, KMT2A_MLLT10 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo
Plasmid#204669PurposeMammalian expression of membrane-bound Pink Flamindo for cAMP imagingDepositorArticleInsertMyr-Pink Flamindo
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q)
Plasmid#170125PurposeLentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signalDepositorInsertMCP-ADAR2-DD(E488Q)-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CaMPARI
Plasmid#60421PurposeExpresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integratorDepositorInsertCaMPARI
UseTagsNuclear Export SignalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
hα1Ga-pDsRed
Plasmid#45811DepositorInserthα1Ga (CACNA1G Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-FilaminA-N-9
Plasmid#54098PurposeLocalization: Actin/Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertFilaminA (FLNA Human)
UseTagsmEmeraldExpressionMammalianMutationD2626HPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
nls-EGFP
Plasmid#67652PurposeMammalian expression vector driving nls-EGFP.DepositorInsertnls-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OSKM
Plasmid#80481PurposepiggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
GPI-mEGFP
Plasmid#182866PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertGPI-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMTS-hFluc
Plasmid#177716PurposeFirefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targetingDepositorInserthFluc
UseTagsMitochondrial Targeting Sequence MTS SSExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
F2R-Tango
Plasmid#66276PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertF2R (F2R Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_TOMM20_sfCherry2(11)
Plasmid#83033PurposeExpresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cellsDepositorInsertTOMM20_sfCherry+11
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbALFA
Plasmid#201484PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertLgBit-NbALFA
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-ABE
Plasmid#195335Purposevector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-ABE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-mCh
Plasmid#80492Purposedonor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expressionDepositorInsertmCherry
UseDonor vector (human)TagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
psfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP
Plasmid#176280PurposeViral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertmNaChBac-P2A-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215326PurposeLentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertdNTP63 Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterdNTP63Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cre)
Plasmid#174863PurposeExpresses human Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by HsPeg10 UTRs
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-loxSTOPlox-LynEGFP
Plasmid#133922PurposeReporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombinationDepositorInsertLynEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-EFS-SaABE8eV106W-bGH-U6-sgRNA-BsmBI
Plasmid#189924PurposeAAV genome encoding SaABE8eV106W and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e V106W
UseAAVTagsExpressionMutationSaCas9 D10A, TadA ABE8e V106WPromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAF123
Plasmid#135921PurposeAll-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.DepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS2-YFP
Plasmid#27122DepositorInsertMS2
UseTagsEYFP, HA, and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only