-
Plasmid#220906PurposeMobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)DepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT011
Plasmid#225162PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT008
Plasmid#225159PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT007
Plasmid#225158PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (AmpR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT014
Plasmid#225165PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT013
Plasmid#225164PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (SmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT012
Plasmid#225163PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (GmR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT010
Plasmid#225161PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT009
Plasmid#225160PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (TetR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT006
Plasmid#225157PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT005
Plasmid#225156PurposeLow copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT001
Plasmid#225152PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT002
Plasmid#225153PurposeHigh copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA324
Plasmid#214741PurposeLow copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSV015
Plasmid#214738PurposeLow copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validationDepositorInsertBBa_J23108-RBS(7504)-thyA-RBS(4427)-infA
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro
Plasmid#211468PurposeLentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminalDepositorInsert4xGFP11-SEC61B
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSGKP_AcsgRNA
Plasmid#203807PurposepSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.DepositorInsertAcgRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-mRFP
Plasmid#170982PurposeEncodes sgRNA scaffold with mRFP in place of target and Kanamycin resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.DepositorInsertmRFP
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS193
Plasmid#197143PurposescCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS138
Plasmid#197142PurposescCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationEGFP(M1V)PromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only