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Showing: 4261 - 4280 of 5104 results

  3. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

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  4. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171803-plasmid-map-sequence-id-341955

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  5. pCRISPaint-mScarlet-F-gB-BlastR [M1G]

    Plasmid
    #171816
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-gB-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171816-plasmid-map-sequence-id-338124

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  6. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

    Plasmid
    #171812
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-gB-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171812-plasmid-map-sequence-id-337985

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  7. pCRISPaint-mNeon-F-gB-BlastR [M1G]

    Plasmid
    #171807
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1G
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-F-gB-T2A-BlastR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171807-plasmid-map-sequence-id-337979

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  14. pDONR223_SERPINB5_WT_V5

    Plasmid
    #83002
    Purpose
    Gateway Donor vector containing SERPINB5, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Matthew Meyerson, Jesse Boehm, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    SERPINB5 (SERPINB5 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    S176P; AA190-192delTDTins25;AA194-196delPVQinsSIP…
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    83002-plasmid-map-sequence-id-162775

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  18. pDONR223_GTPBP8_WT_V5

    Plasmid
    #82979
    Purpose
    Gateway Donor vector containing GTPBP8, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Matthew Meyerson, Jesse Boehm, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    GTPBP8 (GTPBP8 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82979-plasmid-map-sequence-id-162787

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  19. pDONR223_FAM173B_WT_V5

    Plasmid
    #82986
    Purpose
    Gateway Donor vector containing FAM173B, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Matthew Meyerson, Jesse Boehm, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    FAM173B (FAM173B Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    T75M
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82986-plasmid-map-sequence-id-162906

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  20. pDONR223_DNAJC15_WT_V5

    Plasmid
    #82964
    Purpose
    Gateway Donor vector containing DNAJC15, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Matthew Meyerson, Jesse Boehm, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    DNAJC15 (DNAJC15 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    R35G
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82964-plasmid-map-sequence-id-162694

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Showing: 4261 - 4280 of 5104 results