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Showing: 8521 - 8560 of 156664 results

  5. pMT-sox10-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80062
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80062-plasmid-map-sequence-id-417618

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  10. pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE

    Plasmid
    #196677
    Purpose
    AAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).
    Depositor
    Haining Zhong
    Article
    Ma et al Nature. 2022 Nov;611(7937):762-768. doi:
    Insert
    PKIα-P2A-mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    196677-plasmid-map-sequence-id-385124

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  11. pUltra-Plus

    Plasmid
    #161775
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest
    Depositor
    Jorge Luis Arias-Arias
    Type
    Empty backbone
    Use
    Lentiviral; Multicistronic
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161775-plasmid-map-sequence-id-316822

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  12. EBFP2-H2B-6

    Plasmid
    #55243
    Purpose
    Localization: Nucleus/Histones, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55243-plasmid-map-sequence-id-159436

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  14. pLEX_306

    Plasmid
    #41391
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41391-plasmid-map-sequence-id-229869

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  16. pD649-HAsp-CD300LD-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156539
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    CD300LD (CD300LD Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    156539-plasmid-map-sequence-id-368391

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  20. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

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  21. pVITRO1-102.1F10-IgM/λ

    Plasmid
    #61880
    Purpose
    Expresses grass pollen allergen Phl p 7 specific human IgM/λ antibody isotype (102.1F10-IgM/λ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    grass pollen allergen Phl p 7 specific human Mu heavy chain expression cassette
    grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 Prom and rEF1 Prom
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61880-plasmid-map-sequence-id-101189

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  22. PB53x EF1-Dendra2-RPB1Amr

    Plasmid
    #81228
    Purpose
    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system
    Depositor
    Timothée Lionnet
    Article
    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1
    Insert
    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    Use
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    N792D alpha amanitin resistance mutation (Bartolo…
    Promoter
    EF1a
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    81228-plasmid-map-sequence-id-394051

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  26. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid
    #208035
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    SUMO1nc (SUMO1 Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Expression
    Mutation
    Q94P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208035-plasmid-map-sequence-id-410209

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  29. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  32. pCRII-BbsI-sgRNAscaffold

    Plasmid
    #159352
    Purpose
    Expression vector for a customizable guide RNA
    Depositor
    Pim Pijnappel
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159352-plasmid-map-sequence-id-314976

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  36. mCherry-Ykt6

    Plasmid
    #209888
    Purpose
    Expresses mCherry tagged Ykt6 in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    Ykt6
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209888-plasmid-map-sequence-id-413665

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Showing: 8521 - 8560 of 156664 results