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pDONR1K18ms

Plasmid

#72644

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Article

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Tags

Expression

Mutation

Promoter

Available since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19-P70a-3WJdB-T

Plasmid

#87311

Purpose

Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Three-way Junction dimeric Broccoli aptamer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti Vp64-dCas9 T2A mTagBFP2

Plasmid

#171052

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Vp64-dCas9 T2A mTagBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_His2-GFP11

Plasmid

#153511

Purpose

His2-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

His2-GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDX_init containing Sb-loop

Plasmid

#132697

Purpose

pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop

Use

M13 phagmid vector

Tags

Expression

Mutation

Promoter

lac

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-His RTN3A1

Plasmid

#31087

Depositor

Jimtong Horng

Article

Tang et al J Biol Chem. 2007 Feb 23. 282(8):5888-98

Insert

reticulon 3 (RTN3 Human)

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pSAC5.02

Plasmid

#16077

Depositor

Jochen Buck, Lonny Levin

Article

Buck et al Proc Natl Acad Sci U S A. 1999 Jan 5. 96

Insert

soluble adenylyl cyclase (Adcy10 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pQTEV-CIP29

Plasmid

#34822

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

cytokine induced protein 29 kDa (SARNP Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D3

Plasmid

#10912

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D3 (CCND3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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TAF3 PHD_pGEX-6P-2

Plasmid

#92100

Purpose

Expresses GST fusion TAF3 PHD domain protein

Depositor

Albert Jeltsch

Article

Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

Insert

TAF3 PHD domain (TAF3 Human)

Use

Tags

GST Tag

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO OM-eLIR

Plasmid

#189004

Purpose

mitophagy-inducing adaptor protein

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

OM-eLIR

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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R-G324D-(Kin-8)

Plasmid

#28179

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Use

Tags

Expression

Mammalian

Mutation

G324D

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pAC14

Plasmid

#197408

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-mCherry

Plasmid

#66534

Purpose

Expresses mCherry under the control of the Concensus T7 Promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Consensus T7

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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Friend MLV Env YFP

Plasmid

#1820

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Envelope (env MuLV)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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pTR1-GPD-TRPC

Plasmid

#190103

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

Depositor

Perng-Kuang Chang

Article

Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pME-BrainbowTEC

Plasmid

#82405

Purpose

Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Inserts

tdTomatoMYC fusion
EGFP
E2CrimsonHA

Use

Tags

HA and MYC

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Flag-XPA

Plasmid

#22895

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

XPA (XPA Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pBSV2G_PflaB-msfGFPBb

Plasmid

#118231

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven msfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2_163

Plasmid

#199095

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Nili Ostrov, Cultivarium Tools, Charlie Gilbert

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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