Addgene

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p663-UBC-PM-TurboID-V5_IDG-K

Plasmid

#214455

Purpose

Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PM-TurboID-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEPkan-S2

Plasmid

#61601

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAID5.1-N

Plasmid

#145808

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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Tg(lyz:EGFP-Mmu.Rpl10a)

Plasmid

#128838

Purpose

RNA isolation from neutrophils via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-PM-miniTurbo-V5_IDG-K

Plasmid

#214454

Purpose

Gateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PM-miniTurbo-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAID5.1-C

Plasmid

#145809

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.3-C

Plasmid

#145813

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.3-N

Plasmid

#145812

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.2-N

Plasmid

#145810

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.4-N

Plasmid

#145814

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.4-C

Plasmid

#145815

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUBC-paGFP-Dest

Plasmid

#105107

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Use

Tags

paGFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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LTRi

Plasmid

#15913

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Osr1

Plasmid

#26485

Depositor

Paul Danielian

Article

So et al Mech Dev. 1999 Jun . 84(1-2):157-60.

Insert

Osr1 (Osr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pAID5.2-C

Plasmid

#145811

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMR150

Plasmid

#32292

Depositor

Verne Chapman, Rudolf Jaenisch

Article

Sanford et al Genes Dev. 1987 Dec;1(10):1039-46.

Insert

genomic methylation probe-minor satellite repeat

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pUBN-paGFP-Dest

Plasmid

#105108

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Use

Tags

paGFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-kif18b-Xbp1-24xPP7

Plasmid

#74931

Purpose

Translation reporter with Xbp1 pause site

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b-Xbp1-24xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74932

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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TLD_009

Plasmid

#19695

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge