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pINTO-N3::hTET3 HxD

Plasmid

#136365

Purpose

Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

Depositor

Roberto Bonasio

Article

Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

Insert

TET3 (TET3 Human)

Use

Tags

2xStrepTagII, FLAG, and HA

Expression

Mammalian

Mutation

HxD (H1077Y, D1079A)

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD2-m1

Plasmid

#114120

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Use

Tags

His6 and bdSUMO

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-human OCT4 DE-SV40-Luc

Plasmid

#52414

Purpose

Luciferase reporter plasmid for DE OCT4 enhancer activity

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

Distal element of human OCT4 promoter (POU5F1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-2P-Kv

Plasmid

#179462

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-JEDI-2P-Kv-WPRE

Plasmid

#179465

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKII

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Insert

JEDI-2P-Kv

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha2-EGFP

Plasmid

#160976

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-alpha3-EGFP

Plasmid

#160977

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform alpha3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNTK Gatad2a flox

Plasmid

#110812

Purpose

Donor targeting vector for generating mouse Gatad2a conditional knockout allele

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta1-EGFP

Plasmid

#160978

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/TO-eGFP-REV7

Plasmid

#114128

Purpose

Expresses N-terminally tagged eGFP-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 15, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pT2-shATRX

Plasmid

#124258

Purpose

Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shATRX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA_FRT_SNCA-Avi_HygR-2A-BirA.dna(TO_deleted). (pEHA1448)

Plasmid

#209083

Purpose

SNCA (Avi tagged) expression along with BirA ligase

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

SNCA (SNCA Human)
BirA

Use

Tags

Avi

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta3-EGFP

Plasmid

#160980

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta3 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta3 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-FHA

Plasmid

#114129

Purpose

Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

RNF8 FHA domain (amino acids 2-160) (RNF8 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

aa 2-160

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pGLUE-SHLD2

Plasmid

#114118

Purpose

Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Tags

HA tag, Streptavidin-binding tag (Streptag), and …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

Plasmid

#209084

Purpose

Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
BirA

Use

Tags

Expression

Mammalian

Mutation

human codon optimized

Promoter

hSyn

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for deletion)

Plasmid

#110814

Purpose

Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for flox targeting)

Plasmid

#110815

Purpose

Used with pNTK Gatad2a flox allele targeting construct in mouse cells

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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