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Showing: 4221 - 4240 of 19168 results

  1. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

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  3. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

    Plasmid
    #208047
    Purpose
    Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    RNF4 (RNF4 Human)
    Use
    Lentiviral
    Tags
    BirAopt
    Expression
    Mutation
    Cys>Ala mutations in conserved RING domain
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208047-plasmid-map-sequence-id-410205

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  4. TFORF0704

    Plasmid
    #143726
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR2 (NCOR2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    143726-plasmid-map-sequence-id-409056

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  5. pLIX403-ccdB-EYFP

    Plasmid
    #158564
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158564-plasmid-map-sequence-id-309879

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  6. sgRNA.SFFV.tBFP

    Plasmid
    #169940
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.
    Depositor
    Benjamin Ebert
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    169940-plasmid-map-sequence-id-338359

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  8. TFORF2700

    Plasmid
    #142978
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ESRRG (ESRRG Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142978-plasmid-map-sequence-id-427320

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  9. TFORF2651

    Plasmid
    #144058
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TERB1 (TERB1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144058-plasmid-map-sequence-id-400556

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  12. LentiGuide-Puro-NCOA7g1 (BB22)

    Plasmid
    #139457
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139457-plasmid-map-sequence-id-308472

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  13. LentiGuide-Puro-NCOA7g2 (BB23)

    Plasmid
    #139458
    Purpose
    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker
    Depositor
    Caroline Goujon
    Article
    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.
    Insert
    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a / U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139458-plasmid-map-sequence-id-308461

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Showing: 4221 - 4240 of 19168 results