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Showing: 4221 - 4240 of 4262 results

  2. BM00654

    Plasmid
    #155339
    Purpose
    Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.
    Depositor
    Matias Zurbriggen
    Article
    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi
    Insert
    RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    155339-plasmid-map-sequence-id-360426

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  5. pGAPTrap-Luc2-IRESMeo

    Plasmid
    #82509
    Purpose
    Vector targeting the Firefly Luciferase (Luc2) gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Meo gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    Luc2-IRESMeo
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    82509-plasmid-map-sequence-id-156578

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  12. pMY-Ly6G-P2A-PuroR-HA-Rluc_miR

    Plasmid
    #163340
    Purpose
    Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection marker
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    Ly-6G (Ly6g Mouse)
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Availability
    Academic Institutions and Nonprofits only
    163340-plasmid-map-sequence-id-322050

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  13. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid
    #163359
    Purpose
    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.
    Depositor
    Louis Ates, Teunis Geijtenbeek
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    Ly-6G (Ly6g Mouse)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Availability
    Academic Institutions and Nonprofits only
    163359-plasmid-map-sequence-id-322106

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  15. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid
    #165976
    Purpose
    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pphlO6), Synthetic promoter (…
    Availability
    Academic Institutions and Nonprofits only
    165976-plasmid-map-sequence-id-328850

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  16. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid
    #165977
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Availability
    Academic Institutions and Nonprofits only
    165977-plasmid-map-sequence-id-328856

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  19. pcDNA5/FRT/TO-GAlphai1-RLuc8

    Plasmid
    #140973
    Purpose
    Encodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai1-RLuc8 (GNAI1 Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    140973-plasmid-map-sequence-id-300655

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  20. pdCas9-M-C4

    Plasmid
    #73427
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Availability
    Academic Institutions and Nonprofits only
    73427-plasmid-map-sequence-id-135327

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Showing: 4221 - 4240 of 4262 results