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Showing: 4221 - 4240 of 5448 results

  2. GFP-DroshaS300E/S302D

    Plasmid
    #62531
    Purpose
    Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid
    Depositor
    Bharat Ramratnam
    Article
    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-
    Insert
    human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    changed serine 300 to glutamic acid and serine 3…
    Promoter
    Available since
    March 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid
    #155051
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Jason Moffat, Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155051-plasmid-map-sequence-id-308437

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  4. PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

    Plasmid
    #187273
    Purpose
    Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K
    Depositor
    Alpha Yap
    Article
    Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.
    Insert
    Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Anillin A740D, E758K
    Promoter
    U6, CMV
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    187273-plasmid-map-sequence-id-373245

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  6. pCIneo-AR-S81E

    Plasmid
    #171224
    Purpose
    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Glu (AR-S81E)
    Depositor
    Steven Balk
    Article
    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10
    Insert
    human androgen receptor Ser81Glu mutant (AR Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    AR-Ser81Glu (AR-S81E)
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171224-plasmid-map-sequence-id-336020

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  15. pIgG1-2.1

    Plasmid
    #225831
    Purpose
    Mouse IgG1 chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG- and Strep2-tagged monoclonal antibodies for use as tag-specific ELISA standards.
    Depositor
    Gabriel Victora
    Article
    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:
    Insert
    Mouse anti-CGG, IgG1 heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225831-plasmid-map-sequence-id-447625

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  17. TLNRD1(4H)-2T_pET47b

    Plasmid
    #224069
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    L191T/A225T double mutant; reduces affinity of TL…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224069-plasmid-map-sequence-id-443086

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  18. TLNRD1(4H)-2E_pET151

    Plasmid
    #224068
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS; V5
    Expression
    Bacterial
    Mutation
    K192E/R233E double mutant; prevents actin binding…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224068-plasmid-map-sequence-id-443085

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Showing: 4221 - 4240 of 5448 results