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Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUG-Pten
Plasmid#140732PurposeLentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion proteinDepositorInsertGFP-Pten (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhUbiCAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1
Plasmid#58432PurposeInduces Citrine expression from a promoter containing 1 lexA box in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)1-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Mito-Car-GECO
Plasmid#100765PurposeLentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imagingDepositorInsertLenti-Mito-Car-GECO
UseLentiviralTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1: E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_WiChR_TS_mScarlet_ER
Plasmid#205997PurposeHigh efficient, K+ selective channelrhodopsin for the inhibition of neuronsDepositorInsertWobblia Inhibitory Channelrhodopsin
UseAAVTagsTS, mScarlet, ERExpressionMutationPromoterhSynAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vimentin-7
Plasmid#55156PurposeLocalization: Intermediate Filaments, Excitation: 587, Emission: 610DepositorInsertVimentin (VIM Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
ND6-DdCBE-left side TALE
Plasmid#157842Purposeexpresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFR-PATagRFP
Plasmid#31950DepositorInsertPATagRFP
UseTagsEGF receptor (EGFR)ExpressionMammalianMutationR69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…PromoterCMVAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tom20-V5-FKBP-AP_pLX304
Plasmid#120914PurposeLentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membraneDepositorInsertTom20-V5-FKBP-AP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSLBP_mNG_FS_Donor
Plasmid#191387PurposeDonor for C-terminal tagging of endogenous hSLBPDepositorInsertmNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneUseTagsHis6-MBP-prescissionExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJR103
Plasmid#187242PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LIC
Plasmid#26094PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
hHS1-M7A
Plasmid#159170PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1-M7A
UseTagsExpressionMammalianMutationMutated Met 7 of the cyt b562 module of hHS1 to A…PromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDG458
Plasmid#100900PurposeSpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAGExpressionMammalianMutationPromoterCBh and U6Available sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
ITGAV-bio-His
Plasmid#51919PurposeExpresses full-length Integrin alpha-V precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITGAV (ITGAV Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF1499
Plasmid#143505PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRBL1 (RBL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro FLAG SREBP2
Plasmid#32018DepositorInsertSREBP2 (Srebf2 Mouse)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBwt-PURO
Plasmid#208045PurposeEnables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycinDepositorInsertUb (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationPromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-SpCas9-sgRNA
Plasmid#179582PurposeExpresses spCas9 and gRNA that targets ColE1 plasmids for curingDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC8A1_STOP
Plasmid#161328PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC8A1 (SLC8A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-Akt DN (K179M)
Plasmid#16243DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHAExpressionMammalianMutationDominant negative (K179M)PromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-mIFNa2
Plasmid#135098PurposeProtein expression of mouse IFNa2 in mammalian cellsDepositorInsertmIFN-α2 (Ifna2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xHA-TurboID_pAS31
Plasmid#118220Purposeexpresses 3xHA-tagged TurboID in C. elegans intestineDepositorInsertTurboID (BirA mutant)
UseTags3x HA tagExpressionWormMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterges-1pAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3 Flag p38 alpha (agf)
Plasmid#20352DepositorInsertpDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationReplace Thr 180 and Tyr 182 with Ala and Phe resp…PromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX302 EGFP-V5
Plasmid#141348PurposeExpresses V5 tagged GFPDepositorInsertEGFP
UseLentiviralTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-EGFP
Plasmid#50473PurposeEGFP under the control of GFAP promoterDepositorInsertEGFP
UseAAVTagsN/AExpressionMutationPromoterGFAPAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pML104-KanMx4
Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMal_Abl-PRM 3R
Plasmid#112086PurposeBacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.DepositorInsertAbl-PRM-3R (ABL1 Human)
UseTagsHis-6 and MBPExpressionBacterialMutationconstruct contains only the PRM motif of Abl1PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK7-HA-S6K1-E389-deltaCT
Plasmid#8993DepositorInsertS6K1 (Rps6kb1 Rat)
UseTagsHAExpressionMammalianMutationT389E, stop codon at amino acid 402PromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
TFORF1845
Plasmid#143325PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertALX4 (ALX4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3507
Plasmid#144983PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNOCT (NOCT Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-MFF
Plasmid#141158PurposeDimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membraneDepositorInsertmitochondrial fission factor (MFF Human)
UseTagsGBExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB1
Plasmid#63102PurposeExpression of HSPB1 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb1 (HSPB1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b
Plasmid#80612PurposepBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_CHIKV_E1E2
Plasmid#207261PurposeMammalian expression of Chikungunya virus E1E2 proteinDepositorInsertCHIKV_E1E2 (CHIKVgp2 Chikungunya virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCyterm-mScarlet3_N1
Plasmid#189778PurposeConstruct for testing oligimerization of mScarlet3 in mammalian cells by OSER assayDepositorInsertCyterm-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti_gRNA-Puro
Plasmid#84752PurposeExpresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Taq DNA Pol
Plasmid#178782PurposeInducible expression of Taq DNA polymerase in E. coliDepositorInsertTaq DNA Polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only